Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transfer of small RNA from extracellular vesicles secreted by primary human airway epithelial cells to Pseudomonas aeruginosa

ABSTRACT: P. aeruginosa were exposed to extracellular vesicles (EVs) secreted by primary human airway epithelial cells from 3 donors or PBS vehicle control for 6 h. Total RNA was isolated, small RNA libraries were prepared with the QIAseq smRNA kit (Qiagen) and 75-bp single-end reads were generated using Illumina MiniSeq. To assess transfer of human EV small RNAs to P. aeruginosa, sequences that did not map to the PA14 reference genome were aligned to the human genome.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE174710 | GEO | 2021/07/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transfer of small RNA from extracellular vesicles secreted by primary human airway epithelial cells to Pseudomonas aeruginosa

Project description:P. aeruginosa were exposed to extracellular vesicles (EVs) secreted by primary human airway epithelial cells from 3 donors or PBS vehicle control for 6 h. Total RNA was isolated, small RNA libraries were prepared with the QIAseq smRNA kit (Qiagen) and 75-bp single-end reads were generated using Illumina MiniSeq. To assess transfer of human EV small RNAs to P. aeruginosa, sequences that did not map to the PA14 reference genome were aligned to the human genome.

2023-04-11 | GSE228919 | GEO

small RNA-Seq showing transfer of bacterial outer membrane vesicle (OMV) sRNA to host airway epithelial cells

Project description:This study is the first to show transfer of regulatory bacterial sRNAs from bacterial OMVs to host cells. Demonstration of transfer of bacterial sRNA from Pseudomonas aeruginosa OMVs to host cells in two cell types. RNA isolated from PA14 OMV exposed primary human bronchial epithelial cells or CFBE41o- bronchial epithelial cells as well as unexposed control cells was analyzed by small RNA-Seq. Primary cell exposures were performed on two donors and exposures of CFBE41o- cells were done in triplicate.

2016-05-13 | E-GEOD-80421 | biostudies-arrayexpress

An rRNA fragment in extracellular vesicles secreted by human airway epithelial cells increases the fluoroquinolone sensitivity of P. aeruginosa

Project description:Extracellular vesicles (EVs) secreted by human airway epithelial cells contain small RNAs that are transferred to P. aeruginosa upon EV exposure. The purpose of this experiment was to characterize the effect of a 27-nucleotide long human 18S rRNA external transcribed spacer fragment on P. aeruginosa protein levels.

2023-10-24 | PXD033165 | Pride

Using small RNA-Seq to identify differential small RNA content of bacterial outer membrane vesicles (OMVs) secreted by Pseudomonas aeruginosa in response to tobramycin exposure

Project description:Characterization of the sRNA content of OMVs harvested from Pseudomonas aeruginosa strain PA14 LB cultue with and without tobramycin (1ug/mL)

2021-09-12 | GSE183895 | GEO

P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil

2008-11-12 | GSE9592 | GEO

Transcription profiling of P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil

Project description:Comparative transcriptome analyses of P. aeruginosa PA14 pyrF mutant with PA14 wild type, and with PA14 pyrF mutant with 1 mM uracil supplement and PA14 wild type with 10 mM uracil in biofilm cells. All samples were cultured in LB with glass wool at 37C for 7h. Experiment Overall Design: Strains: P. aeruginosa PA14 wild type, PA14 pyrF mutant Experiment Overall Design: Medium: LB for PA14 wild type and PA14 pyrF mutant, LB with 1 mM uracil for PA14 pyrF mutant, LB with 10 mM uracil for PA14 wild type Experiment Overall Design: Cell type: Biofilm cells grown on glass wool Experiment Overall Design: Time: 7 h Experiment Overall Design: Temperature: 37C

2008-11-14 | E-GEOD-9592 | biostudies-arrayexpress

Within-species control reveals novel immune response genes in Caenorhabditis elegans

Project description:Used P. aeruginosa z11 strain as a control for P. aeruginosa PA14 infection studies in C. elegans

2023-08-31 | GSE205337 | GEO

P. aeruginosa PA14 mutant PA4496 biofilm vs wild-type biofilm

Project description:P. aeruginosa PA14 mutant strain PA4496 expression in biofilm cells relative to PA14 wild-type strain expression in biofilm cells. All samples cultured in LB with glass wool

2018-02-14 | GSE24638 | GEO

Genomic DNA hybridization of Pseudomonas aeruginosa strains PAO1 and PA14

Project description:Genomic DNA from Pseudomonas aeruginosa strains PAO1 and PA14

2015-11-15 | GSE67038 | GEO

microRNA repertoire of wild-type C57BL/6 mice T cell blasts and their secreted small extracellular vesicles (Evs)

Project description:T cell blasts were obtained from wild-type C57BL/6 mice. Six-day culture blast supernatants were ultracentrifuged for small EV obtention and small EV pellet obtained after from 100,000g ultracentrifugation and T cell blasts RNA were isolated for small RNA sequencing; (N=3) independent experiments

2020-01-20 | GSE140982 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data