Similar Datasets
Project description:Study of host response in mice to uropathogenic E. coli (UPEC) infection and the contribution of deletion of the Cnf and HlyA1 toxins to the host response. Mice were infected with UPEC in the bladder for 24 hrs and RNA was isolated from whole bladder to analyze on Agilent whole mouse 2-channel arrays against normal untreated mouse bladder RNA. Biological replicates were analyzed for each condition. Infection: genotype of E. coli CP9 (UPEC) strain
2013-05-14 | GSE37953 | GEO
Project description:Study of host response in mice to uropathogenic E. coli (UPEC) infection and the contribution of deletion of the Cnf and HlyA1 toxins to the host response. Mice were infected with UPEC in the bladder for 24 hrs and RNA was isolated from whole bladder to analyze on Agilent whole mouse 2-channel arrays against normal untreated mouse bladder RNA. Biological replicates were analyzed for each condition. Infection: genotype of E. coli CP9 (UPEC) strain strain_or_line_design
2013-05-14 | E-GEOD-37953 | biostudies-arrayexpress
Project description:Study of host response in mice to uropathogenic E. coli (UPEC) infection and the contribution of deletion of the Cnf and HlyA1 toxins to the host response. Mice were infected with UPEC in the bladder for 24 hrs and RNA was isolated from whole bladder to analyze on Agilent whole mouse 2-channel arrays against normal untreated mouse bladder RNA. Biological replicates were analyzed for each condition. Infection: genotype of E. coli CP9 strain strain_or_line_design
2013-02-06 | E-GEOD-37959 | biostudies-arrayexpress
Project description:Study of host response in mice to uropathogenic E. coli (UPEC) infection and the contribution of deletion of the Cnf and HlyA1 toxins to the host response. Mice were infected with UPEC in the bladder for 24 hrs and RNA was isolated from whole bladder to analyze on Agilent whole mouse 2-channel arrays against normal untreated mouse bladder RNA. Biological replicates were analyzed for each condition. Infection: genotype of E. coli CP9 strain
2013-02-06 | GSE37959 | GEO
Project description:Murine macrophages in mice infected with UTI
| PRJNA731357 | ENA
Project description:Recurrent urinary tract infections (rUTI) are a costly clinical problem affecting millions of women worldwide each year. The majority of rUTI cases are caused by uropathogenic Escherichia coli (UPEC). Data from humans and mouse models indicate that some instances of rUTI are caused by UPEC emerging from latent reservoirs in the bladder. Some studies have reported that women with vaginal dysbiosis, typically characterized by high levels of Gardnerella vaginalis and other anaerobes, are at increased risk of UTI. Multiple studies have detected G. vaginalis in urine collected by transurethral catheterization (to limit vaginal contamination), suggesting that some women experience routine urinary tract exposures. We recently reported that inoculation of G. vaginalis into the bladder triggers rUTI from UPEC bladder reservoirs in a mouse model. Here we performed whole bladder RNAseq to identify host pathways involved in G. vaginalis-induced rUTI. We identified multiple host pathways differentially expressed following G. vaginalis exposure. At the gene and transcript level, we identified upregulation of the orphan nuclear receptor Nur77 (aka Nr4a1) and Nur77-regulated genes. Pilot data from Nur77 knockout mice suggests that Nur77 is necessary for G. vaganalis exposure to trigger rUTI.
2021-11-01 | GSE186800 | GEO
Project description:Either WT or muMT KO mice were infected with UPEC trans urethrealy twice 1 hour apart and culled 6 hours later. Kidneys were subsequently perfused, RNA extracted and analysed via RNASeq
2022-11-01 | GSE139848 | GEO
Project description:Antimicrobial peptides (AMPs) serve key proposed roles in defending the urinary tract against invading uropathogens, but individual AMPs bearing greatest responsibility for these functions remain largely unknown. We identified RegIIIγ as the most transcriptionally upregulated AMP in bladder transcriptomes following uropathogenic Escherichia coli (UPEC) infection. We confirmed induction of RegIIIγ mRNA during cystitis and pyelonephritis by quantitative RT-PCR. Immunoblotting demonstrates increased bladder and urinary RegIIIγ protein levels following UPEC infection. Immunostaining localizes RegIIIγ protein to urothelial cells of infected bladders and kidneys. Human patients with cystitis and pyelonephritis exhibit increased urine levels of the orthologous HIP/PAP protein. Recombinant RegIIIγ protein does not demonstrate bactericidal activity toward UPEC in vitro, but does kill Staphylococcus saprophyticus in a dose-dependent manner. RegIIIγ knockout and control urinary tracts contain comparable bacterial burden following experimental inoculation of UPEC as well as Gram-positive uropathogens. Thus, while RegIIIγ and HIP/PAP expression occurs in human and murine UTI, their specific functions in the urinary tract remain uncertain.
2015-12-29 | GSE72007 | GEO
Project description:Either WT or PI3KδE1020K-B KI mice were infected with UPEC trans urethrealy twice 1 hour apart and culled 12 hours later. Kidneys were subsequently perfused, RNA extracted and analysed via RNASeq
2022-11-01 | GSE139849 | GEO
Project description:Transcriptional analysis of UTI89 - uropathogenic E.coli (UPEC) strain grown in urine/Luria bertani medium culture in vitro as well as during three distinct phases of UPEC bladder infection: intracellular growth, filament formation and filament reversal. UTI89 was used to infect a bladder epithelial cell line cultured within a dynamic flow chamber system and harvested at particular stages of its pathogenecity cascade. Total RNA was processed and cy3 labeled for microarray analysis using Agilent custom Escherichia coli UTI89 arrays designed using E-Array.
2017-05-28 | E-MTAB-3611 | biostudies-arrayexpress