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Evalutation of DEGs by RNA-seq in panobinostat-exposed (5 mg/kg/day) embryonic murine ovary

ABSTRACT: We hypothesized that gestational exposure (E6.5-E15.5) to panobinostat, a HDAC inhibitor, can lead to alteration of murine ovary transcriptome. To test our hypothesis, we exposed mice (C57Bl6j) to 5 mg/kg/day of panobinostat during embryonic development (E5.5-E15.5). Four embryonic ovaries were pooled prior RNA extraction using RNeasy Plus kit. RNA libraries (3 control, 3 exposed) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina. After pre-processing of the sequencing data we performed an analysis of differentially expressed genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE174831 | GEO | 2021/08/06

REPOSITORIES: GEO

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