Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [amplicon-seq]

ABSTRACT: we uncover a key transcriptional network involving hundreds of genes that is required for HSC self-renewal.

ORGANISM(S): Homo sapiens

PROVIDER: GSE175518 | GEO | 2022/11/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [smart-seq2]

Project description:we uncover a key transcriptional network involving hundreds of genes that is required for HSC self-renewal.

2022-11-23 | GSE175520 | GEO

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [10X Genomics]

Project description:We uncover a key transcriptional network involving hundreds of genes that is required for HSC self-renewal.

2022-11-23 | GSE175516 | GEO

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [RNA-Seq]

Project description:we uncover a key transcriptional network involving hundreds of genes that is required for HSC self-renewal.

2022-11-23 | GSE216225 | GEO

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [bulk RNA-seq]

Project description:we uncover a key transcriptional network involving hundreds of genes that is required for HSC self-renewal.

2022-11-23 | GSE175515 | GEO

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [ChIP-Seq]

Project description:The molecular regulation of human hematopoietic stem cell (HSC) maintenance is therapeutically important, but limitations in experimental systems and interspecies variation have constrained our knowledge of this process. Here, we have studied a rare genetic disorder due to MECOM haploinsufficiency, characterized by an early-onset absence of HSCs in vivo. By generating a faithful model of this disorder in primary human HSCs and coupling functional studies with integrative single-cell genomic analyses, we uncover a key transcriptional network involving hundreds of genes that is required for HSC maintenance. Through our analyses, we nominate cooperating transcriptional regulators and identify how MECOM prevents the CTCF-dependent genome reorganization that occurs as HSCs differentiate. Strikingly, we show that this transcriptional network is co-opted in high-risk leukemias, thereby enabling these cancers to acquire stem cell properties. Collectively, we illuminate a regulatory network necessary for HSC self-renewal through the study of a rare experiment of nature.

2022-11-23 | GSE214399 | GEO

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia.

Project description:The molecular regulation of human hematopoietic stem cell (HSC) maintenance is therapeutically important, but limitations in experimental systems and interspecies variation have constrained our knowledge of this process. Here, we have studied a rare genetic disorder due to MECOM haploinsufficiency, characterized by an early-onset absence of HSCs in vivo. By generating a faithful model of this disorder in primary human HSCs and coupling functional studies with integrative single-cell genomic analyses, we uncover a key transcriptional network involving hundreds of genes that is required for HSC maintenance. Through our analyses, we nominate cooperating transcriptional regulators and identify how MECOM prevents the CTCF-dependent genome reorganization that occurs as HSCs differentiate. We show that this transcriptional network is co-opted in high-risk leukemias, thereby enabling these cancers to acquire stem cell properties. Collectively, we illuminate a regulatory network necessary for HSC self-renewal through the study of a rare experiment of nature.

| S-EPMC9810535 | biostudies-literature

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-11-23 | GSE175521 | GEO

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [amplicon-seq]

Project description:A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [amplicon-seq]

| PRJNA732774 | ENA

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia

Project description:A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia

| PRJNA732690 | ENA

A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [ChIP-Seq]

Project description:A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia [ChIP-Seq]