Single cell RNA sequencing data of T cells co-cultured with tumor cells
Ontology highlight
ABSTRACT: We developed a photosensitive tag (PsT) to label cells that reside in specific areas of interest in (human) primary tissues through specific exposure with violet light, allowing analysis of single cells while preserving their spatial information. As a benchmarking experiment, we used the PsT to assess transcriptional differences between CD8+ T cells that reside in an area containing tumor cells that do express cognate antigen, or an area of tumor cells that do not express the antigen. Single cell RNA sequencing revealed differential expression of activation markers between CD8+ T cells from antigen-positive or antigen-negative areas, demonstrating the value of the PsT to investigate spatially-induced differences between cells residing in primary tissues.
ORGANISM(S): Homo sapiens
PROVIDER: GSE175813 | GEO | 2021/05/31
REPOSITORIES: GEO
ACCESS DATA