Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of housekeeping sortase disruption on Bifidibacterium bifidum toranscriptome

ABSTRACT: To explore the molecular mechanism of B. bifidum adhesion, we compared transcriptomes among WT and non-adhesive mutants cultured in MRS or m-ILS medium.

ORGANISM(S): Bifidobacterium bifidum

PROVIDER: GSE175843 | GEO | 2021/11/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Functional genomic analyses of Bifidobacterium bifidum PRL2010 highlight how this microorganism interact with the human host

Project description:Bifidobacteria represents one of the dominant group of microorganisms colonizing the intestine of infants. However, the genetic determinants supporting the establishment and the interaction with the human hosts are still largely unknown. Most commensal bacteria interacting with eukaryotic hosts express adhesive molecules on their surfaces that modulate interaction with host cell receptors or with soluble macromolecules. Whole genome transcription profiling of B. bifidum PRL2010, a strain isolated from infant stool, under in vitro as well as in vivo conditions revealed the expression of few common extracellular proteins among which type 1 pili encoding genes. To investigate the molecular mechanisms sustaining the interaction of PRL2010 strain with the human gut, we first explored the global genome transcription profiling of this strain in a in vitro human model such as in the presence of HT29 cell lines. The transcriptome was analyzed using a custom B. bifidum PRL2010 array representing the 90% of this organismM-bM-^@M-^Ys protein coding genes. To better evaluate the conserved responses by B. bifidum, the in vivo transcriptomes were quantified against a diverse set of transcriptome patterns identified for in vitro laboratory cultures of the strain, i.e., B. bifidum responses after growth on an cellM-bM-^@M-^Ys monolayers growth medium (DMEM); B.bifidum responses after growth on synthetic medium (MRS). Briefly, we analized five conditions, two of which are also used as references. Every experiment was performed in duplicate and in vivo condition was performed in quadruplicate.

2013-07-01 | E-GEOD-36442 | biostudies-arrayexpress

Effects of housekeeping sortase disruption on Bifidibacterium bifidum toranscriptome

Project description:Effects of housekeeping sortase disruption on Bifidibacterium bifidum toranscriptome

| PRJNA734023 | ENA

Effects of Bifidobacterium bifidum PRL2010 on gene expression in intestinal epithelial cells

2010-10-01 | GSE21976 | GEO

Effects of Bifidobacterium bifidum PRL2010 on gene expression in intestinal epithelial cells

Project description:Analysis of gene expression in Caco-2 intestinal epithelial cells stimulated with Bifidobacterium bifidum PRL2010. We used microarrays to investigate gene expression in intestinal epithelial cells in response to Bifidobacterium bifidum PRL2010, in particular genes involved in mucin pathways. Caco-2 cells were grown in transwell plates to 4 days post-confluence. Cells were then incubated for 2h and 4h with Bifidobacterium bifidum PRL2010. The experiment was performed in duplicate. Caco-2 RNA was extracted and hybridized to Affymetrix NuGO_Hs1a52018 arrays.

2010-10-01 | E-GEOD-21976 | biostudies-arrayexpress

Functional genomic analyses of Bifidobacterium bifidum PRL2010 highlight how this microorganism interact with the human host

2013-07-01 | GSE36442 | GEO

Bifidobacterium bifidum

Project description:Bifidobacterium bifidum pangenome

| PRJNA263318 | ENA

Bifidobacterium bifidum

Project description:Bifidobacterium bifidum Genome sequencing

| PRJNA640518 | ENA

Bifidobacterium bifidum

Project description:Bifidobacterium bifidum Genome sequencing

| PRJNA1015039 | ENA