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Otx ChIP-chip on Ciona intestinalis embryo (early gastrula)

ABSTRACT: A genomic overview of in vivo binding of a transcription factor Otx in the ascidian early gastrula embryo.

ORGANISM(S): Ciona intestinalis

PROVIDER: GSE17690 | GEO | 2010/03/09

SECONDARY ACCESSION(S): PRJNA123633

REPOSITORIES: GEO

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Otx ChIP-chip on Ciona intestinalis embryo (early gastrula)

Project description:A genomic overview of in vivo binding of a transcription factor Otx in the ascidian early gastrula embryo. Fertilized eggs were electroporated with a construct encoding GFP-tagged Otx. After fixation, chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE (Whole Cell Extract) DNA were amplified by LM-PCR for the Chip analysis. Two independent experiments were performed to reduce experimental errors.

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