Project description:Comparison of LAPC cells isolated from naive PBS treated and influenza treated mice. LAPC cells from treated and untreated mice. No replicates. Comparison of expression signatures of LAPC cells with other immune system cells.
Project description:MC38 tumors from C57BL/6 mice treated with GB2 or PBS were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, MRNA was purified from total RNA using polyT and then fragmented with 10x RNA fragmentation buffer and the RNA-seg library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced.
Project description:We sorted macrophages (known to be infected by influenza viruses from the lungs of mice infected with MA-Venus-PR8 on the basis of their fluorescent protein expression and performed microarray analysis. Macrophages isolated from the lungs of mice inoculated with PBS (naive macrophages) served as controls.
Project description:We sorted macrophages (known to be infected by influenza viruses from the lungs of mice infected with MA-Venus-PR8 on the basis of their fluorescent protein expression and performed microarray analysis. Macrophages isolated from the lungs of mice inoculated with PBS (naive macrophages) served as controls. 15 samples
Project description:Disruption of blood-brain barrier (BBB) integrity is a hallmark of several neurological disorders. Here we show that the BBB is dynamically and differentially affected during the preclinical, progression and remission phase of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). We isolated vascular fragments, representing the BBB, from spinal cords of imatinib and PBS treated mice at the preclinical, progression and remission phase of MOG-EAE as well as from non-immunized, naive mice.
Project description:DNA microarray experiments were used to compare gene expression profiles of untreated and 5-azacytidine treated Escherichia coli at both logarithmic phase and early stationary phase The goal was to determine the effect of cytosine DNA methylation loss on gene expression (5-azacytidine is a methylation inhibitor)
Project description:To identify gene expression changes associated with treatment of EVs from MDA-MB-231 [231] and MCF10A [10A] cells) in NIH3T3 cells, we analyzed RNA isolated from PBS- or EV-treated NIH3T3 cells. Gene expression in NIH3T3 cells treated with EV from MDA-MB-231 cells was compared to cells treated with PBS or EV from MCF10A cells, both of which served as controls in this experiment.