Methylation profiling

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Identification of methylated sites in BT-549 cells affected by thymoquinone treatment


ABSTRACT: BT-549 cells were treated with thymoquinone for 6 h and chromatin immune-precipitation (ChIP) was performed using anti- MBD1 antibody (Abcam, USA). Anti-MBD1 could precipitate specific genomic region, which are methylated. The ChIP products were sequenced by library construction and bioinformatics analysis. The gene ontology (GO) analysis for multiple pathways was performed. The genomic methylated regions affected by TQ treatment were identified.

ORGANISM(S): Homo sapiens

PROVIDER: GSE178334 | GEO | 2022/06/15

REPOSITORIES: GEO

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