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The impact of RNAi on the Saccharomyces castellii transcriptome

ABSTRACT: RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and Y´ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y´ mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.

ORGANISM(S): Naumovozyma castellii

PROVIDER: GSE17870 | GEO | 2009/09/10

SECONDARY ACCESSION(S): PRJNA123553

REPOSITORIES: GEO

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The impact of RNAi on the Saccharomyces castellii transcriptome

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