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Single-cell transcriptional analyses reveal tissue-driven segregation of T cell function in secondary antiviral response

ABSTRACT: Virus-specific memory T cells generated during primary infection and maintained in tissues and circulation are capable of initiating a quick and robust response upon secondary exposure to viral antigens. To understand the nature of this recall response and the factors that determine antiviral T cell function, we performed single-cell transcriptome profiling of virus-specific CD8+ T cells across diverse tissue sites following stimulation with viral antigens using pooled library amplification for transcriptome expression sequencing (PLATE-seq)(Bush et al., 2017). From this analysis, we found that antigen-responsive cells primarily cluster based on tissue and not virus specificity, with the exception of CMV-specific T cells from the BM. These findings suggest tissue-regulated antiviral T cell function, with specific CMV-driven influences in the BM.

ORGANISM(S): Homo sapiens

PROVIDER: GSE178837 | GEO | 2022/01/05

REPOSITORIES: GEO

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