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The brain endothelial cell translatome analysis in the visual cortex at plastic and nonplastic ages

ABSTRACT: In this study, we provide the translatome data in the brain endothelial cells (BECs) of the visual cortex at 3 different time points (pre-critical period (P10), critical period (P30), post-critical period (P100)) by using translating ribosome affinity purification (TRAP) combined with high-throughput RNA sequencing analysis in Tie2-Cre:Ribo-Tag mice, which are designed for expressing hemagglutinin A (HA)-tagged RPL22, a ribosomal protein in endothelial cells depending on CRE recombinase. To obtain a high-quality BEC pool, we modified the TRAP method by adding the capillary isolation process before the immunoprecipitation step and finally verified BEC-specific translating mRNA purification using RT-PCR.

ORGANISM(S): Mus musculus

PROVIDER: GSE178918 | GEO | 2025/02/06

REPOSITORIES: GEO

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