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Total RNA sequencing of mouse LSK cells in E14.5 fetal liver

ABSTRACT: The study is to obtain a transcriptome-wide map of prenatal murine stem and progenitor cells. VavCre+ mice were used for conditional knockout of Ddx41. Wild-type, Ddx41 heterozygous (Ddx41+/-) and Ddx41 knockout (Ddx41-/-) LSK cells from embryonic 14.5 day fetal liver were harvested (three samples from each group) for RNA. Gene expression analysis and alternative splicing analysis were performed. RNA-seq data were confirmed by RT-qPCR and regular PCR followed by gel quantification. A global change of gene expression and substantial differential splicing events were observed in KO samples compared to WT and het.

ORGANISM(S): Mus musculus

PROVIDER: GSE178979 | GEO | 2022/02/04

REPOSITORIES: GEO

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Total RNA sequencing of mouse LSK cells in E14.5 fetal liver

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