Dataset Information

Antioxidants in culture and vitrification media alters mouse liver and placenta gene expression

ABSTRACT: A multitude of factors encompassing assisted reproductive technologies present compounding stresses and cause synergistic damage to cell function and embryo development in vitro. We have previously reported that antioxidant supplementation of culture and vitrification media ameliorate some of the detrimental effects of in vitro culture and cryopreservation. The aim of this study was to identify the molecular mechanisms by which antioxidants improve embryo and fetal development by comparing mRNA profiles of E14.5 post-implantation fetal (liver) and placental tissue following supplementation of either in vitro culture or vitrificiation media with or without antioxidants. In vitro samples are compared with tissues derived from in vivo derived fetuses and placentae.

ORGANISM(S): Mus musculus

PROVIDER: GSE179114 | GEO | 2021/12/01

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Background: Since the development of in vitro embryo production in cattle, different supplements have been added to embryo culture media to support development, with serum being the most popular. However, the addition of serum during embryo culture can induce high birth weights and low viability in calves (Large Offspring Syndrome). Global gene expression analysis of bovine embryos through RNA sequencing produced in different conditions (in vivo, in vitro with serum added and in vitro without addition of serum) can provide valuable information to optimize culture media for in vitro embryo production, which is necessary to obtain healthy offspring. Results: Here, we used RNA sequencing to examine the effect of in vitro embryo production, either in serum or in serum-free conditions, on the global gene expression pattern of individual bovine blastocysts. For this purpose, we compared in vitro produced with in vivo derived embryos. The embryos produced in serum had ten times more genes differentially expressed than the embryos produced in serum-free conditions (1,106 vs. 110). Importantly, in vitro production appeared to have a different impact on the embryos according to their sex, with male embryos showing more deviations in their gene expression pattern than their female counterparts. In particular, embryos produced in the presence of serum showed the largest difference, with male embryos having eight times more genes differentially expressed than the females (1,561 vs. 200). This difference was reduced when the embryos were produced in serum-free conditions, where the male embryos had only about double the number of genes differentially expressed than their female counterparts (64 vs. 35). The pathways affected by in vitro production differed depending on the supplementation. While embryos produced in serum conditions had altered genes involved in RNA processing and mitosis, embryos produced in serum-free conditions showed aberrations in genes involved in lipid metabolism. Conclusions: Serum supplementation had a major impact on the gene expression pattern of the embryos. On the contrary, the transcriptome of embryos produced in serum-free conditions resembled more in vivo derived embryos, although genes involved in lipid metabolism were altered. Male embryos appeared to be most affected by in vitro production, especially after serum supplementation.

Dataset Information

Antioxidants in culture and vitrification media alters mouse liver and placenta gene expression

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets