Genomics

Dataset Information

0

Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis


ABSTRACT: Background: Leptospirosis, a global zoonotic infectious disease, has various clinical manifestations ranging from mild self-limiting illness to life-threatening with multi-organ damage, including liver involvement. This study was aimed at identifying circulating microRNAs (miRNAs) as novel biomarkers for predicting severe liver involvement in patients with leptospirosis. Methods: In a discovery set, 12 serum samples of patients with anicteric and icteric leptospirosis at initial clinical presentation were used for miRNA profiling by a NanoString nCounter miRNA assay. In a validated cohort, top candidate miRNAs were selected and further tested by qRT-PCR in serum samples of 81 and 16 individuals with anicteric and icteric leptospirosis, respectively. Results: The discovery set identified 38 significantly differential expression miRNAs between the two groups. Among these, miR-601 and miR-630 were selected as the top two candidates significantly up-regulated expressed in the icteric group. The enriched KEGG pathway showed that these miRNAs were mainly involved in immune responses and inflammation. In the validated cohort, miR-601 and miR-630 levels were significantly higher in the icteric group compared with the anicteric group. Additionally, these two miRNAs displayed good predictors of subsequent acute liver failure with a high sensitivity of 100%. On regression analysis, elevated miR-601 and miR-630 expression were also predictive of multi-organ failures and poor overall survival. Conclusion: Our data indicated that miRNA expression profiles were significantly differentiated between the icteric and anicteric groups. Serum miR-601 and miR-630 at presentation could potentially serve as promising biomarkers for predicting subsequent acute liver failure and overall survival in patients with leptospirosis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179426 | GEO | 2021/07/07

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-02-14 | GSE72199 | GEO
2020-12-22 | GSE153581 | GEO
2020-12-22 | GSE153580 | GEO
2012-12-01 | E-GEOD-25019 | biostudies-arrayexpress
2016-02-14 | E-GEOD-72199 | biostudies-arrayexpress
2012-12-01 | GSE25019 | GEO
2014-11-28 | GSE59565 | GEO
2015-10-31 | GSE70754 | GEO
2021-07-15 | GSE179961 | GEO
2022-01-31 | GSE185062 | GEO