Transcriptomics

Dataset Information

0

Cilium induction triggers differentiation of glioma stem cells


ABSTRACT: Glioblastoma multiforme (GBM) possesses glioma stem cells (GSCs) that promote self-renewal, tumor propagation, and relapse. Understanding the mechanisms of GSCs self-renewal can offer targeted therapeutic interventions. However, insufficient knowledge of the fundamental biology of GSCs is a significant bottleneck hindering these efforts. Here, we show that patient-derived GSCs recruit an elevated level of proteins that ensure the temporal cilium disassembly, leading to suppressed ciliogenesis. Depleting the cilia disassembly complex components at the ciliary base is sufficient to induce ciliogenesis in a subset of GSCs via sequestering PDGFR-α from its original location to newly induced cilium. Importantly, restoring ciliogenesis caused GSCs to behave like healthy NPCs switching from self-renewal to differentiation. Finally, using an organoid-based glioma invasion assay and brain xenografts in mice, we establish that ciliogenesis-induced differentiation can prevent the infiltration of GSCs into the brain. Our findings illustrate a crucial role for cilium as a molecular switch in determining GSCs' fate and suggest that cilium induction is an attractive strategy to intervene in GSCs proliferation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179439 | GEO | 2021/07/08

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-08-01 | GSE273250 | GEO
2024-08-01 | GSE273252 | GEO
2012-12-20 | E-GEOD-39486 | biostudies-arrayexpress
| PRJNA743803 | ENA
2023-09-28 | GSE185695 | GEO
2018-11-28 | GSE123017 | GEO
2024-10-15 | GSE255535 | GEO
2019-04-04 | GSE112677 | GEO
2021-03-24 | GSE169418 | GEO
2017-09-01 | GSE75224 | GEO