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A novel NGS-based method can improve the efficiency and sensitivity of Y2H screening with cDNA library

ABSTRACT: The NGS-based Y2H screening methods have been adopted to increase the efficiency and sensitivity, while reducing the labor and experimental cost of the canonical Y2H screening. However, most of NGS-based Y2H screening methods are suitable for well-constructed ORFeomes but not cDNA libraries. Thus, we developed a novel NGS-based Y2H screening method to accomplish a precise Y2H screening with cDNA libraries. With newly designed primers, we can distinguish and filter out those non-in-frame reads from all mapped reads, which facilitates the estimation of the interaction intensity between baits and preys.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE179488 | GEO | 2021/07/09

REPOSITORIES: GEO

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