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RNA-seq of neuroblastoma syngeneic mouse models treated with anlotinib

ABSTRACT: We utilized murine neuroblastoma (NB) cell lines 975A2 and 9464D. Both cell lines were derived from spontaneous NB in the adrenal glands of TH-MYCN transgenic mice on a C57BL/6 background. 975A2 (2 x 106) and 9464D (1 x 106) tumor cells were subcutaneously implanted into the right back of 6-week-old C57BL/6 mice. When the mean tumor volume reached 30-50 mm3, the mice were randomized into individual treatment groups. After 9 days of anlotinib (6mg/kg) treatment, 3 mice from the treatment group and the vehicle group were sacrificed, and the tumors were snap-frozen with liquid nitrogen immediately after resection. Sequencing was performed at Beijing Novogene Co., Ltd., and the differentially expressed genes (DEGs) were subjected to enrichment analysis. The results showed that anlotinib treatment significantly upregulated immune-related pathways such as adaptive immune response, T cell activation, regulation of INF-γ production, lymphocyte chemotaxis, migration and adhesion, and cytokine receptor activity and significantly downregulated angiogenesis-related pathways such as cellular response to vascular endothelial growth factor stimulus and blood vessel endothelial cell proliferation involved in sprouting angiogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE179550 | GEO | 2021/11/25

REPOSITORIES: GEO

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