Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Detection call p-values for Affymetrix low level spike-in experiment

ABSTRACT: Four different concentrations (0, 0.75, 1.5, 3 pM) of genes were spiked in groups (group size 6-7 transcripts) Each concentration combination were spiked into a common backgroung sample (HeLa cell line) and hybridized to three HG-U133A Plus 2.0 Arrays. Keywords: Latin square design for HG-U133A Plus 2.0 GeneChips

ORGANISM(S): Homo sapiens

PROVIDER: GSE17968 | GEO | 2009/11/10

SECONDARY ACCESSION(S): PRJNA119941

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of human samples for detection call p-values for Affymetrix low level spike-in experiment

Project description:Four different concentrations (0, 0.75, 1.5, 3 pM) of genes were spiked in groups (group size 6-7 transcripts); Each concentration combination were spiked into a common backgroung sample (HeLa cell line) and hybridized to three HG-U133A Plus 2.0 Arrays. Experiment Overall Design: Latin square design Experiment Overall Design: List of probe sets belonging to each spike-in concentration group. Experiment Overall Design: Probe set Spike Group Experiment Overall Design: 204205_at J Experiment Overall Design: 204265_s_at J Experiment Overall Design: 204951_at J Experiment Overall Design: 205790_at J Experiment Overall Design: 205903_s_at J Experiment Overall Design: 207641_at J Experiment Overall Design: 207655_s_at J Experiment Overall Design: 203508_at K Experiment Overall Design: 204912_at K Experiment Overall Design: 204959_at K Experiment Overall Design: 205692_s_at K Experiment Overall Design: 206060_s_at K Experiment Overall Design: 209795_at K Experiment Overall Design: 210895_s_at K Experiment Overall Design: 203471_s_at L Experiment Overall Design: 204836_at L Experiment Overall Design: 205267_at L Experiment Overall Design: 207540_s_at L Experiment Overall Design: 209734_at L Experiment Overall Design: 212827_at L Experiment Overall Design: 200665_s_at M Experiment Overall Design: 201721_s_at M Experiment Overall Design: 204563_at M Experiment Overall Design: 205249_at M Experiment Overall Design: 205671_s_at M Experiment Overall Design: 212886_at M

2009-11-14 | E-GEOD-17968 | biostudies-arrayexpress

Homo sapiens

Project description:Affymetrix HG-U133A 2.0 Expression data

| PRJNA127807 | ENA

Affymetrix HG-U133A 2.0 Expression data

Project description:Expression .CEL files from Affymetrix HG-U133A 2.0 arrays using DNA from 14 human cell lines derived from metastasized melanoma

2010-07-08 | GSE22787 | GEO

Gene expression data on human keratinocytes with knockdown or overexpression of SNAI2

Project description:1. Keratinocytes infected with retroviruses expressing control or SNAI2 shRNAs were cultured in growth medium and Affymetrix HG-U133 plus 2.0 arrays were used to determine global gene expression profiles. 2. Keratinocytes infected with retroviruses overexpressing LACZ or SNAI2 were cultured in growth medium and Affymetrix HG-U133 plus 2.0 arrays were used to determine global gene expression profiles.

2014-10-15 | GSE55269 | GEO

Performance Evaluation of Commercial miRNA Expression Array Platforms

Project description:This data is part of a miRNA platform comparison study. We compared the performance characteristics of four commercial miRNA array technologies and found that all platforms performed well in separate measures of performance. The Ambion and Agilent platforms were more accurate, whereas the Illumina and Exiqon platforms were more specific. Furthermore, the data analysis approach had a large impact on the performance, predominantly by improving precision. Performance of four (4) commercially available miRNA platforms was evaluated using 7 placenta samples spiked with synthetic microRNA spikes (in Latin-square design) absent in placenta. Platforms were primarily evaluated for accuracy and specificity.

2010-06-23 | E-GEOD-19248 | biostudies-arrayexpress

Distinct phenotype and function of anergic CD8+ T cells produced by Treg-cell suppression.

Project description:Four conditions of cultured CD8+ T cells were analyzed with Affymetrix HG-U133-Plus-2.0 microarrays.

2014-11-11 | GSE63129 | GEO

caArray_EXP-608: TCGA (Laml): Analysis of RNA Expression Array Data for Acute Myeloid Leukemia Using Affymetrix HG-U133 Plus 2.0 (WUSTL)

Project description:TCGA Analysis of RNA Expression Array Data for Acute Myeloid Leukemia Using Affymetrix HG-U133 Plus 2.0

2015-05-14 | GSE68833 | GEO

Expression profiling of neuroblastic tumors

Project description:The expression profiles of 64 neuroblastic tumors (mainly neuroblastoma) were determined on Affymetrix chips HG U133 Plus 2.0.

2008-10-16 | GSE12460 | GEO

LS_SpikedSerum

Project description:Latin square spiked serum

| PAe000262 | PeptideAtlas

HCaRG-9 vs NEO-1

Project description:HEK293 cells were transfected with control plasmid (pcDNAI/Neo;Invitrogen) or with the plasmid encoding HCaRG. Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen). Chips HG-U133A: - Labeling protocol: Enzo BioArray HighYield RNA Transcript Labeling Kit. - Hybridization: According to the manufacturer's protocol (Affymetrix). - Scanner: GeneArray 2500. - Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500. Chips HG-U133 Plus 2.0: - Labeling protocol: GeneChip IVT Labeling Kit. - Hybridization: According to the manufacturer's protocol (Affymetrix). - Scanner: GeneChip Scanner 3000. - Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500. Keywords: parallel sample

2005-10-19 | GSE2555 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data