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Transcriptome-wide maps of m6A RNA methylation marks in murine liver

ABSTRACT: N6-methyladenosine (m6A), the most prevalent modification of mRNAs at the (G/A)(m6A)C motif installed by METTL3 that plays critical role in regulation of gene expression. METTL3 is essential for embryonic development, and its dysregulation is linked to various diseases including metabolic disorders and cancer. Here, we provide insight into this by generating Mettl3fl/fl; Alb-Cre (M3LKO) mice carrying a hepatocyte-specific deletion of Mettl3. RNA-seq and MeRIP-seq analyses of mRNA identified significant dysregulation of metabolic genes in M3LKO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE179680 | GEO | 2021/09/15

REPOSITORIES: GEO

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Liver Homeostasis, Hepatocyte Ploidy, and Circadian Rhythm-controlled Gene Expression are Perturbed in Liver-Specific METTL3 Knockout Mice

Project description:METTL3 is essential for ESC differentiation and embryonic development, and its dysregulation is linked to various diseases including metabolic disorders and cancer. However, the consequence of m6A perturbation in the mammalian liver is not understood. Here, we provide insight into this by generating mice M3LKO (Mettl3fl/fl; Alb-Cre) mice carrying a hepatocyte-specific deletion of Mettl3.

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2023-08-18 | PXD036899 | Pride

The RNA N6-methyladenosine regulates chromatin remodeling and transcription [mESC_seq_RNA_mNET]

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