Transcriptomics

Dataset Information

0

CD151 expression indicates the subtype specification of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes


ABSTRACT: Current differentiation protocols for human pluripotent stem cells produce a heterogeneous population of cardiomyocytes (CMs). Here, we identified CD151 as a marker of ventricular CMs (VCMs) and atrial CMs (ACMs) from 212 different cell surface markers. In the VCM induction, CD151high CMs were a homogeneous population of mature VCMs, including binuclear VCMs, and showed enriched cell cycle-related genes based on RNA-seq analysis. As for the ACM induction, CD151low CMs expressed high levels of atrial-related genes and exhibited atrial-type electrophysiological properties. According to RNA-seq analysis, CD151high CMs from the ACM induction had molecular signatures for cell-cell interactions and NOTCH signaling. When treated with a NOTCH signal inhibitor, the same cells showed mature electrophysiological properties consistent of ACMs with an increasing expression of atrial-related genes. Altogether, we found that CD151 is an indicator of subtype specification with distinct mechanisms between VCM and ACM differentiation and that NOTCH signaling inhibition enhances atrial specification.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179769 | GEO | 2024/01/29

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-01-19 | GSE239317 | GEO
2024-01-19 | GSE239321 | GEO
2024-10-01 | GSE236870 | GEO
2017-12-31 | GSE100199 | GEO
2024-08-09 | PXD038300 | Pride
2023-08-11 | GSE237090 | GEO
2023-08-11 | GSE222579 | GEO
2023-08-11 | GSE222373 | GEO
2024-09-24 | GSE255991 | GEO
2024-09-24 | GSE255612 | GEO