Transcriptomics

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Identification of H3K27ac-marked enhancers in bovine satellite cells during differentiation


ABSTRACT: In this study we isolated satellite cells from skeletal muscle from two Angus-crossbred steers, initially cultured them in growth medium for proliferation, and then in differentiation medium to induce them to differentiate and fuse into myotubes. We used ChIP-seq to identify enhancers marked with H3K27ac, i.e., active enhancers, from these cells immediately before and two days after induction of differentiation. Nearly 20,000 and 50,000 H3K27ac-marked enhancers were consistently identified from two biological replicates of before- and during-differentiation bovine satellite cells, respectively.

ORGANISM(S): Bos taurus

PROVIDER: GSE179821 | GEO | 2021/12/23

REPOSITORIES: GEO

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