Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

10X single-cell RNASeq profiling of human in vitro differentiated Th17 cell [scRNA-seq]

ABSTRACT: We performed single-cell RNAseq to study the role of ZEB1 in human Th17 cells differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179831 | GEO | 2021/07/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

10X single-cell RNASeq profiling of human in vitro differentiated Th17 cell [scRNA-seq]

Project description:10X single-cell RNASeq profiling of human in vitro differentiated Th17 cell [scRNA-seq]

| PRJNA745206 | ENA

Transcriptome analysis studying the role of ZEB1 in Th1 and Th17 differentiation [RNA-seq]

Project description:We performed bulk-RNAseq to study the role of ZEB1 in human and mouse Th1 and Th17 cells differentiation.

2021-07-12 | GSE179832 | GEO

Expression data from in vitro versus in vivo differentiated Th17 cells

Project description:In vitro differentiated Th17 have a distinct expression profile compared to in vivo differentiated Th17 Several conditions of Th17 cells were tested, with 2-3 replicates per condition.

2015-04-18 | E-GEOD-68017 | biostudies-arrayexpress

in vitro differentiated Th0, Th17, and Tr1 cells

Project description:Gene expression profiling of in vitro differentiated murine Th cell subsets. Flow cytometrically sorted naive Th cells (CD4+ CD44- Foxp3-) were polyclonally stimulated in vitro for 3 days using 4 Âµg/ml plate-bound antibody to CD3 (145-2C11) and 2 Âµg/ml soluble antibody to CD28 (PV-1). Th0 cells were cultured in the absence of exogenous cytokines. Th17 cells were differentiated with 50 ng/ml IL-6 plus 0.5 ng/ml TGF-Î². Tr-1 cells were differentiated with 100 ng/ml IL-27 plus 0.5 ng/ml TGF-Î². Five biological replicates per Th subset (Th0, Th17, Tr1)

2014-03-20 | E-GEOD-56021 | biostudies-arrayexpress

Single-cell transcriptional profiling of Th17 cells, differentiated in vitro for 48h [TGFB1_IL6-48h]

Project description:Extensive cellular heterogeneity exists within specific immune-cell subtypes classified as a single lineage, but its molecular underpinnings are rarely characterized at a genomic scale. Here, we use single-cell RNA-seq to investigate the molecular mechanisms governing heterogeneity and pathogenicity of Th17 cells isolated from the central nervous system (CNS) and lymph nodes (LN) at the peak of autoimmune encephalomyelitis (EAE) or polarized in vitro under either pathogenic or non-pathogenic differentiation conditions. Computational analysis reveals a spectrum of cellular states in vivo, including a self-renewal state, Th1-like effector/memory states and a dysfunctional/senescent state. Relating these states to in vitro differentiated Th17 cells, unveils genes governing pathogenicity and disease susceptibility. Using knockout mice, we validate four novel genes: Gpr65, Plzp, Toso and Cd5l (in a companion paper). Cellular heterogeneity thus informs Th17 function in autoimmunity, and can identify targets for selective suppression of pathogenic Th17 cells while sparing non-pathogenic tissue-protective ones. Single-cell transcriptional profiling of Th17 cells, differentiated in vitro for 48h

2015-11-20 | E-GEOD-75110 | biostudies-arrayexpress

Expression data from in vitro versus in vivo differentiated Th17 cells

Project description:In vitro differentiated Th17 have a distinct expression profile compared to in vivo differentiated Th17

2015-04-18 | GSE68017 | GEO

Single-cell transcriptional profiling of Th17 cells, differentiated in vitro for 48h [TGFB1_IL6-48h]

2015-11-20 | GSE75110 | GEO

Single-cell transcriptional profiling of Th17 cells, differentiated in vitro for 48h [IL1B_IL6_IL23-48h-IL-17A/GFP+]

2015-11-20 | E-GEOD-75109 | biostudies-arrayexpress

Single-cell transcriptional profiling of Th17 cells, differentiated in vitro for 48h [TGFB1_IL6-48h-IL-17A/GFP+]

2015-11-20 | E-GEOD-75111 | biostudies-arrayexpress

RNA sequencing on in vitro differentiated WT and GOT1-/- Th17 cells

Project description:Naive CD4+ T cells (CD4+CD25-CD44-CD62L+) were FACS sorted from WT or T cell conditional GOT1-/- C57BL/6 mouse spleen and lymph nodes. Sorted cells were differentiated into Th17 cells in the presence of TGFβ, IL1β, IL6, IL23, anti-IFNγ and anti-IL4. RNA sequencing was then performed on day4 differentiated TH17 cells.

2023-02-02 | GSE215115 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data