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A longitudinal DNA methylation dataset in primary human fibroblasts with mitochondrial genetic, metabolic, and endocrine perturbations

ABSTRACT: The current DNA methylation dataset (n=479) provides an epigenetic map of cultured primary human fibroblasts (in vitro) aged through the cellular lifespan. The dataset was generated using the Illumina EPIC bead array, which includes DNA methylation levels (0-1.0) at 866,091 CpG sites. This provides DNA methylation trajectories collected at high temporal frequency (every 8 days, ~7timepoints per a donor) across the entire replicative lifespan of primary human fibroblast cell lines from n=6 healthy male and female donors and n=3 donors with lifespan-altering mitochondrial disease caused by mutations in the SURF1 gene. The dataset can be integrated with other measures collected in parallel along the lifespan, including cytological (cell size, morphology), bioenergetic (energy expenditure, derived ATP synthesis rates from Seahorse), gene expression (RNA sequencing, GSE179848), secreted proteins, telomere length, and whole-genome sequencing (WGS) data. This dataset also includes experimental manipulations with treatments targeting oxidative phosphorylation (OXPHOS) and glycolysis, and glucocorticoid signaling, providing an opportunity to examine the influence of stress and bioenergetics on human aging biology. All processed data can be accessed and browsed at our webtool: https://columbia-picard.shinyapps.io/shinyapp-Lifespan_Study/

ORGANISM(S): Homo sapiens

PROVIDER: GSE179847 | GEO | 2021/11/12

REPOSITORIES: GEO

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