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Influence of IWS1 TND-interacting motifs (TIMs) on chromatin localization patterns [ChIP-seq]

ABSTRACT: To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we performed ChIP using anti-FLAG antibodies from these samples and performed chromatin occupancy profiling using ChIP-seq. Analysis of locations reveal that both WT and M3 samples are highly enriched in at transcribed gene bodies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179909 | GEO | 2021/11/26

REPOSITORIES: GEO

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