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Global chromatin relabeling accompanies spatial inversion of chromatin in rod photoreceptors

ABSTRACT: We used CUT&RUN sequencing technology for high-throughput profiling of histone modifications in murine rod photoreceptor cells during post-natal maturation. We generated genome-wide chromatin-state maps of mouse embryonic stem cells, and rod cells isolated from post-natal days 1, 7, 14, 21, and adult mice. We find that Histone H3 Lysine 9 methylation status changes over the course of rod cell maturation, accompanying the transition of nuclear architecture from "conventional" to "inverted" such that H3K9me2-labeled nuclear peripheral heterochromatin is relabeled with H3K9me3 and repositioned to the nuclear center, while transcriptionally active euchromatin is labeled with H3K9me2 and positioned at the nuclear periphery. Global chromatin relabeling is correlated with spatial rearrangement, suggesting a critical role for histone modifications, specifically H3K9 methylation, in nuclear architecture.

ORGANISM(S): Mus musculus

PROVIDER: GSE180006 | GEO | 2021/09/29

REPOSITORIES: GEO

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Global chromatin relabeling accompanies spatial inversion of chromatin in rod photoreceptors

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