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Microenvironment analysis of mouse and human neuroblastoma reveals shared populations and unmasks various subsets of immunosuppressive cells

ABSTRACT: High-risk neuroblastoma is a pediatric cancer with dismal prognosis. In this study, we have used various approaches including single-cell RNA sequencing to dissect the tumor microenvironment of both a transgenic mouse neuroblastoma model and a cohort of biopsies from neuroblastoma patients. From the diverse cell populations identified, our data document a striking correspondence of the microenvironment populations between tumors from both species and unravel a complex content in myeloid cells and cancer-associated fibroblasts. Within the myeloid compartment we have characterized a population of cells exhibiting features of tumor-associated neutrophils and demonstrate that these cells obtained from the mouse model have immunosuppressive properties. Altogether, our study shows that neuroblastoma tumors have an immunocompromised microenvironment characterized by dysfunctional T-cells and accumulation of immunosuppressive cells. Our data provide the rationale for novel immunotherapeutic strategies in neuroblastoma, targeting both tumor cells and components of its microenvironment.

ORGANISM(S): Mus musculus

PROVIDER: GSE180101 | GEO | 2023/02/02

REPOSITORIES: GEO

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Microenvironment analysis of mouse and human neuroblastoma reveals shared populations and unmasks various subsets of immunosuppressive cells

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