Genomics

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Sequencing of siRNA from soybean containing silencing vectors and empty vectors


ABSTRACT: A cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score > 5, reads containing N < 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed

ORGANISM(S): Glycine max

PROVIDER: GSE180131 | GEO | 2021/07/21

REPOSITORIES: GEO

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