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Transcriptomic analyis of in-vitro plasma cell generation from follicular and marginal zone B cells with cell cycle inhibition via PD0332991 and rapamycin

ABSTRACT: Plasma cell differentiation is thought to be tied to cell division. We observe that marginal zone B cells are able to differentiate to plasma cells without completing a single cell division. In order to characterize the transcriptome of plasma cells derrived from un-divided MZ B cells we performed in vitro plasma cell differentiation cultures in the presence of the CDK4/6 inhibitor PD0332991 or rapamycin using Blimp1-GFP reporter follicular and MZ B cells. We report here that these resulting plasma cells from undivided MZ B cells exhibit normal plasma cell gene expression and function in the absence of mitosis.

ORGANISM(S): Mus musculus

PROVIDER: GSE180170 | GEO | 2021/07/22

REPOSITORIES: GEO

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