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Inhibition of EZH2 with GSK343 in the LIMe-Hi-C K562 clone

ABSTRACT: We characterized the the effect of EZH2 inhibition on gene expression in the LIMe-Hi-C K562 clone

ORGANISM(S): Homo sapiens

PROVIDER: GSE180229 | GEO | 2022/06/17

REPOSITORIES: GEO

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Inhibition of EZH2 with GSK343 in the LIMe-Hi-C K562 clone

Project description:Inhibition of EZH2 with GSK343 in the LIMe-Hi-C K562 clone

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The development and application of LIMe-Hi-C to simultanteously study lamina association, DNA methylation and chromosome conformation at baseline and upon EZH2 and DNMT1 inhibition

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Full proteome analysis of WT and Ezh2-KO K562 cells

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Phytoplasma-responsive small RNAs in Mexican lime trees

Project description:Background: WitchesM-bM-^@M-^Y broom disease of Mexican lime (Citrus aurantifolia L.), which is caused by the phytoplasma M-bM-^@M-^\Candidatus Phytoplasma aurantifoliaM-bM-^@M-^], is a devastating disease that results in significant economic losses. Plants adapt to abiotic stresses by regulating gene expression at the transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) are a recently identified family of molecules that regulate plant responses to environmental stresses through post-transcriptional gene silencing. Methods: Using a high-throughput approach to sequence small RNAs, we compared the expression profiles of miRNAs in healthy Mexican lime trees and in plants infected with M-bM-^@M-^\Ca. Phytoplasma aurantifoliaM-bM-^@M-^]. Results: Our results demonstrated the involvement of different miRNAs in the response of Mexican lime trees to infection by M-bM-^@M-^\Ca. Phytoplasma aurantifoliaM-bM-^@M-^]. We identified miRNA families that are expressed differentially upon infection with phytoplasmas. Most of the miRNAs had variants with small sequence variations (isomiRs), which are expressed differentially in response to pathogen infection. Conclusions: It is likely that the miRNAs that are expressed differentially in healthy and phytoplasma-infected Mexican lime trees are involved in coordinating the regulation of hormonal, nutritional, and stress signalling pathways, and the complex interactions between them. Future research to elucidate the roles of these miRNAs should improve our understanding of the level of diversity of specific plant responses to phytoplasmas. Small mRNA profiles of healthy (H) and Phytoplasma-infected Mexican lime trees were generated by deep sequencing, six replicate, using Illumina Hiseq2000

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