Alteration of LncRNA expression in mice placentae after frozen embryo transfer is associated with increased fetal weight.

ABSTRACT: Background: Frozen embryo transfer (FET) significantly upregulates fetal weight compared with fresh embryo transfer. However, the mechanism of FET increasing fetal weight remains unclear. Methods: We transferred blastocysts which had been vitrifified and warmed or fresh blastocysts into individual pseudopregnant recipients (n=11 mice each group) produced by natural mating to eliminate the influence of superovulation. The fetal weight, placental weight, placental efficiency and placenta architecture were compared. We performed placental lncRNA sequencing and mRNA sequencing to study the placental transcriptoms changes. And 15 selected differentially expressed lncRNAs and 15 mRNAs was checked. Findings: Enhanced birthweight (1.513 g vs. 1.685 g, P <0.001) was found in FET mice, with increased placental efficiency (11.355 vs. 10.312, P =0.045) and proportion of labyrinth zone area (59.78% vs. 53.19%, P = 0.034), indicating FET might change the placental function and morphology architecture. A total of placental 97608 mRNAs and 103339 lncRNAs were obtained, among which 1012 mRNAs and 554 lncRNAs showed significantly different expression between two groups. KEGG and GO enrichment analyses showed that the differentially expressed lncRNAs and their targeted mRNAs were related to labyrinth zone formation and maternal-fetal interface vascular remodeling. qRT-PCR, Western blotting and correlation analysis demonstrated that suppressed Lncenc1 to elevate Gjb5 might play a role in increased fetal weight of FET. Interpretation: FET changes the placental function and architecture resulting in higher mouse fetal weight, potentially via the network diagram of Lncenc1-miRNA-Gjb5.

ORGANISM(S): Mus musculus

PROVIDER: GSE180310 | GEO | 2022/12/22

REPOSITORIES: GEO