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Global gene expression profiles among five independent cytoplasmic male sterile lines.

ABSTRACT: We employed a microarray-based transcriptomic status comparison of rice cytoplasmic male sterile (CMS) lines in order to categorize the nuclear gene expressions upon cytoplasmic substitution. In anthers at the uninucleate and bicellular pollen stages, we found that 8,199 genes significantly changed their expression in at least one of the CMS lines. We categorized the genes into 100 clusters by k-means clustering, and common obvious expression patterns were observed in W11, LD and BT.

ORGANISM(S): Oryza sativa

PROVIDER: GSE18057 | GEO | 2010/08/22

SECONDARY ACCESSION(S): PRJNA119319

REPOSITORIES: GEO

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label-free analysis of male-sterile line 1355A of cotton

Project description:Cotton (Gossypium spp.) is one of the most important economic crops and exhibits yield-improving heterosis in specific hybrid combinations. The genic male-sterility system is the main strategy used for producing heterosis in cotton. To better understand the mechanisms of male sterility in cotton, we carried out label-free quantitative proteomics analysis in the anthers of two near-isogenic lines, the male-sterile line 1355A and the male-fertile line 1355B, the two isogenic lines. These experiments, along with bioinformatics analyses, identified 124 proteins from the anthers at uninucleate pollen stage (stage 8) that were significantly differentially expressed between these two lines.

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Oryza sativa

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| PRJNA396160 | ENA

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