Transcriptome-wide identification of RNA binding protein binding sites using seCLIP-seq
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ABSTRACT: Discovery of interaction sites between RNA-binding proteins (RBPs) and their RNA targets plays a critical role in enabling our understanding of how these RBPs control RNA processing and regulation. Cross-linking and immunoprecipitation (CLIP) provides a generalizable, transcriptome-wide method by which RBP:RNA complexes are purified and sequenced to identify sites of intermolecular contact. By simplifying technical challenges in prior CLIP methods and incorporating the generation of and quantitative comparison against size-matched input controls, the single-end enhanced CLIP (seCLIP) protocol allows for the profiling of these interactions with high resolution, efficiency, and scalability.
ORGANISM(S): Homo sapiens
PROVIDER: GSE180686 | GEO | 2022/02/07
REPOSITORIES: GEO
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