Transcriptomics

Dataset Information

0

Melatonin mitigates Chloroquine-induced defects in porcine immature Sertoli cells


ABSTRACT: Chloroquine (CQ) could function as a lysosomotropic inhibitor to impair the autophagy-lysosome pathway, and is widely used to treat malarial, tumor and recently COVID-19. However, the effect of CQ treatment on porcine immature Sertoli cells (iSCs) remains unclear.;Here we showed that CQ treatment reduced iSCs viability in a dose-dependent manner. CQ treatment (20µM) of iSCs for 36h could elevate oxidative stress, damage mitochondrial function and promote apoptosis. Melatonin (MT) (10nM) could partially rescue CQ-induced phenotypic defects. Transcriptome profiling (RNA-seq) identified 1611 differentially expressed genes (DEGs) (776 up- and 835 down-regulated) (20µM CQ vs. DMSO control group), mainly involved in MAPK cascade, cell proliferation/apoptosis, HIF-1, PI3K-Akt and lysosome signaling pathways. In contrast, MT addition identified only 467 (224 up- and 243 down-regulated) DEGs (CQ+MT vs. DMSO), enriched in cell cycle, regulation of apoptotic process, lysosome and reproduction, respectively.Collectively, CQ treatment could impair porcine iSC viability by deranging apoptosis- and autophagy related signaling pathways, which could be partially rescued by MT supplementation.

ORGANISM(S): Sus scrofa

PROVIDER: GSE180995 | GEO | 2024/07/06

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-07-08 | MTBLS9273 | MetaboLights
2023-09-20 | GSE212122 | GEO
2012-06-30 | E-GEOD-30351 | biostudies-arrayexpress
2020-03-25 | PXD011279 | Pride
| 2626385 | ecrin-mdr-crc
2012-07-01 | GSE30351 | GEO
2024-06-05 | PXD016095 | Pride
2024-07-31 | MTBLS9673 | MetaboLights
2020-07-22 | GSE137231 | GEO
2022-02-27 | GSE153570 | GEO