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An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE181034 | GEO | 2022/02/07

REPOSITORIES: GEO

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FlowSorted.BloodExtended.EPIC: An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

Project description:DNA methylation microarrays have been extensively used for understanding cell type composition in complex tissue samples. Here we expand on existing libraries for reference-based deconvolution of blood DNA methylation data assayed using the Illumina HumanMethylationEPIC array to include 12 different leukocyte subtypes (neutrophils, eosinophils, basophils, monocytes, B cells naïve and memory, CD4+ and CD8+ naïve and memory cells, natural killers, and T regulatory cells). Application of the IDOL algorithm for identifying optimal libraries for the deconvolution of blood-derived DNA methylation data led to to a library consisting of 1200 CpGs. The accuracy of deconvolution estimates obtained using our IDOL-optimized library was evaluated using artificial mixtures with known cellular composition and in samples were both whole-blood DNA methylation data and FACS information were available. We further showcase potential applications of our expanded library using publicly available cancer, aging, and autoimmune disease data sets.

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An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

Project description:An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

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FlowSorted.BloodExtended.EPIC: An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

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