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Lineage tracing and single-cell analysis reveal proliferative Prom1+ tumor-propagating cells and their dynamic cellular transition during liver cancer progression

ABSTRACT: Hepatocellular carcinoma (HCC) is highly heterogeneous, and this heterogeneity contributes to therapeutic resistance and tumor recurrence. The aim of this study was to investigate the role, heterogeneity, and properties of Prom1+ cells in HCC in intact mouse models. We used Prom1C-L/+;Rosa26tdTomato/+ mice to establish two HCC mouse models representing chronic fibrotic HCC and rapid steatosis-related HCC. We performed lineage tracing at different time points post-HCC induction. We ablated the Prom1+ cell population in Prom1C-L/+;Rosa26DTA/+ mice. Single cell RNA-seq (scRNA-seq) was carried out to analyse the lineage properties, heterogeneity, and dynamics of the traced Prom1+ cells in the chronic fibrotic HCC model. Bulk RNAseq was carried out to analyse the change in transcriptome in Prom1 depleted tumor.

ORGANISM(S): Mus musculus

PROVIDER: GSE181515 | GEO | 2021/10/04

REPOSITORIES: GEO

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Prom1-deficiency effects on mouse primary hepatocyte transcriptomes in serum-free condition

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