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A fungal transcription regulator of vacuolar functions modulates Candida albicans interactions with host epithelial cells [RNA-seq]

ABSTRACT: We employed RNA-Seq to identify targets of regulation of the transcription regualtor ZCF8 in nitrogen-starved Candida albicans cells.

ORGANISM(S): Candida albicans

PROVIDER: GSE181653 | GEO | 2021/08/15

REPOSITORIES: GEO

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A fungal transcription regulator of vacuolar functions modulates Candida albicans interactions with host epithelial cells

Project description:We employed RNA-Seq to identify ZCF8-dependent and ZCF8-indepenent transcriptional changes in Candida albicans cells treated with nigericin.

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A fungal transcription regulator of vacuolar functions modulates Candida albicans interactions with host epithelial cells [ChIP-seq]

Project description:We employed ChIP-Seq to identiy direct targets of regulation of the Candida albicans transcription regulator Zcf8p.

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Comparative phenotypic analysis of the major fungal pathogens Candida parapsilosis and Candida albicans

Project description:Abstract: Candida parapsilosis and Candida albicans are human fungal pathogens that belong to the CUG clade in the Saccharomycotina. In contrast to C. albicans, relatively little is known about the virulence properties of C. parapsilosis, a pathogen particularly associated with infections of premature neonates. We describe here the construction of >200 C. parapsilosis strains carrying double allele deletions of transcription factors, protein kinases and species-specific genes. Two independent deletions were constructed for each target gene. Growth in > 40 conditions was tested, including carbon source, temperature, and the presence of antifungal drugs. The phenotypes were compared to C. albicans strains with deletions of orthologous transcription factors. We found that many phenotypes are shared between the two species, such as the role of Upc2 as a regulator of azole resistance. Others are unique. For example, Cph2 plays a role in the hypoxic response in C. parapsilosis and not in C. albicans. We found extensive divergence between the biofilm regulators of the two species. We identified 7 transcription factors and one protein kinase that are required for biofilm development in C. parapsilosis. Only three (Efg1, Bcr1, and Ace2) have similar effects on C. albicans biofilms, whereas Cph2, Czf1, Gzf3 and Ume6 have major roles in C. parapsilosis only. In addition, two transcription factors (Brg1 and Tec1) with well-characterized roles in biofilm formation in C. albicans do not have the same function in C. parapsilosis. We also compared the transcription profile of C. parapsilosis and C. albicans biofilms. Our analysis suggests the processes shared between the two species are predominantly metabolic.

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Project description:To infer transcriptional networks of nitrogen utilization , Candida albicans was exposed to nitrogen starvation for four hours and then offered arginine or BSA as sole nitrogen source. Time-series transcription data was obtained from both the starvation and the feeding periods, and the response to different nitrogen availability was used for network modelling with selected genes.

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Project description:Candida albicans is the most common human fungal pathogen causing mucosal and systemic infections, but human anti-fungal immunity remains poorly defined. Expression profiling of Candida-stimulated human peripheral blood mononuclear cells (PBMCs) provides new insights into Candida-specific host defense mechanisms in humans.

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