Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative Genome Hybridization of Porphyromonas gingivalis strains

ABSTRACT: CGH was used to compare the genomes of a non-invading P. gingivalis strain to the database W83 strain (invader). For hybridization and scanning, TIGR microarrays and protocols were used. The results were independently processed using two different software packages.

ORGANISM(S): Porphyromonas gingivalis

PROVIDER: GSE18169 | GEO | 2011/07/28

SECONDARY ACCESSION(S): PRJNA119521

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comparative Genome Hybridization of Porphyromonas gingivalis strains

Project description:CGH was used to compare the genomes of a non-invading P. gingivalis strain to the database W83 strain (invader). For hybridization and scanning, TIGR microarrays and protocols were used. The results were independently processed using two different software packages. We used P. gingivalis microarrays in comparative genomics to specifically address the P. gingivalis invasive genotype using invasive and non-invasive phenotypes.

2011-07-27 | E-GEOD-18169 | biostudies-arrayexpress

Porphyromonas gingivalis comparative genomic hybridization

Project description:Genotyping studies suggest that there is genetic variability among P. gingivalis strains, however the extent of variability remains unclear, and the regions of variability have only partially been identified. We previously used heteroduplex analysis of the ribosomal operon intergenic spacer region (ISR) to type P. gingivalis strains in several diverse populations, identifying 6 predominant heteroduplex types and many minor ones. In addition we used ISR sequence analysis to determine the relatedness of P. gingivalis strains to one another, and demonstrated a link between ISR sequence phylogeny and the disease-associated phenotype of P. gingivalis strains. The availability of whole genome microarrays based on the genomic sequence of strain W83 has allowed a more comprehensive analysis of P. gingivalis strain variability, using the entire genome. The objectives of this study were to define the phylogeny of P. gingivalis strains using the entire genome, to compare the phylogeny based on genome content to the phylogeny based on a single locus (ISR), and to identify genes that are associated with the strongly disease-associated strain W83 that could be important for virulence. Keywords: Comparative genomic hybridization

2008-10-09 | GSE13128 | GEO

Porphyromonas gingivalis comparative genomic hybridization

2010-05-18 | E-GEOD-13128 | biostudies-arrayexpress

Porphyromonas gingivalis W83:Transcriptome profiling and functional verification of flavodoxin

Project description:The aim of the study was to identify the role of flavodoxin gene in the virulence of P. gingivalis W83.The mRNA profiles of P. gingivalis W83 and flavodoxin mutant strain were generated by deep sequencing, in triplicate, using Illumina sequencing platform. Differential expression analysis of two groups (Three biological replicates per group) was performed using the DESeq R package. In total, 376 genes met the DEGs criteria (194 upregulated genes and 182 down-regulated genes). The KEGG pathway analysis indicated the DEGs were significantly enriched in three KEGG pathways, comprising pathways associated with Peroxisome, Two-component system and Quorum sensing.Importantly, we found that the flavodoxin gene can regulate the type IX secretion system and oxidative stress system in P. gingivalis W83. The knowledge obtained will help us to deeply understand the role of flavodoxin gene in the P. gingivalis W83 genome.

2021-10-24 | GSE186376 | GEO

Effect of deletion of trkA on gene expression of Porphyromonas gingivalis W83

Project description:To investigate the comprehensive function of trkA in Porphyromonas gingivalis W83, we established isogenic trkA deletion strain via homologous recombination and compared the transcriptional alteration between mutant and wild type group through RNA sequencing.

2022-11-07 | GSE210663 | GEO

Porphyromonas gingivalis W83

Project description:Porphyromonas gingivalis W83 Raw sequence reads

| PRJNA883030 | ENA

Porphyromonas gingivalis W83

Project description:Porphyromonas gingivalis W83 Genome sequencing and assembly

| PRJNA938817 | ENA

Porphyromonas gingivalis

Project description:Polyphosphate affects gene expression and viability of Porphyromonas gingivalis W83

| PRJNA106395 | ENA

Porphyromonas gingivalis

Project description:Porphyromonas gingivalis: Control (Wild-type W83) vs. its htrA mutant

| PRJNA108583 | ENA

Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity

Project description:The aim of this study is to investigate the alterations in gene expression in Porphyromonas gingivalis W83 after inoculation in rat oral cavity. P.gingivalis W83 inoculation in rat oral cavity caused inflammatory responses in gingival tissues and destroyed host alveolar bone. Microarray analysis revealed that 42 genes were upregulated, and 22 genes were downregulated in the detected 1786 genes in the inoculated P.gingivalis W83. Products of these upregulated and downregulated genes are mainly related to transposon functions, cell transmembrane transportation, protein and nucleic acid metabolism, energy metabolism, cell division and bacterial pathogenicity.P.gingivalis W83 has a pathogenic effect on host oral cavity. Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile. These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

2015-04-07 | GSE67608 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data