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Progressively overloaded single-cell RNA-seq with cell hashing

ABSTRACT: Barcode-based multiplexing methods can be used to increase throughput and reduce batch effects in large single-cell genomics studies. To evaluate methods for demultiplexing barcode-multiplexed data, we generated a dataset by labeling samples separately with barcode-tagged antibodies, mixing those samples, and progressively overloading a droplet-based scRNA-seq system.

ORGANISM(S): Homo sapiens

PROVIDER: GSE181862 | GEO | 2021/12/01

REPOSITORIES: GEO

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Comparison of different hashing methods on human PBMCs and primary mice tissues

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