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Distinct regulation of gene promoters and enhancers by CXCL4 and TLR8 in human CD14+ monocytes

ABSTRACT: To gain a comprehensive understanding of gene regulation in CXCL4 and TLR8 signaling crosstalk, we treated primary human blood monocytes with CXCL4 and TLR8 ssRNA ligand ORN8L for 6 h and performed transcriptomic analysis via RNA-seq. We observed that CXCL4 interacted with TLR8 ssRNA ligand and triggered inflammatory cytokine storm including IL6, IL12p40, TNF and IFNβ, and pro-fibrotic gene expression and activated NLRP3 inflammasome leading to interleukin-1β (IL-1β) secretion and pyroptosis in human blood monocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE181886 | GEO | 2022/05/13

REPOSITORIES: GEO

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