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Next Generation Sequencing identifies differentially translated genes regulated by PM2.5 exposure in BEAS-2B cells [Ribo-seq]

ABSTRACT: We report the application of Ribosome sequencing for high-throughput profiling of tanslation efficiency in BEAS-2B cells after exposure to PM2.5.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182200 | GEO | 2022/07/27

REPOSITORIES: GEO

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Next Generation Sequencing identifies differentially expressed genes regulated by PM2.5 exposure in BEAS-2B cells [RNA-seq]

Project description:We report the application of RNA-sequencing for high-throughput profiling of transcriptomes in BEAS-2B cells after exposure to PM2.5 for 24 hours..

2022-07-27 | GSE182199 | GEO

Gene expression data from BEAS-2B cells upon exposure to particulate matter (PM2.5) from biomass combustion

Project description:Exposure to PM2.5 affects gene expression of BEAS-2B cells Gene expression microarrays were used to detail changes in the transcriptome upon exposure to PM2.5

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high-throughput sequencing of circRNAs in T-BEAS-2B cells and C-BEAS-2B cells

Project description:We report the differential expression of circRNAs between T-BEAS-2B cells (cadmium-transformed BEAS-2B cells) and C-BEAS-2B cells (passage-matched control BEAS-2B cells) by high-throughput sequencing. T-BEAS-2B cells are BEAS-2B cells transformed by cadmium at 2.0 μM for twenty weeks, and C-BEAS-2B cells are their passage-matched control. RNAs were sequenced on Illumina HiSeq Xten platform in triplicates, and expressions of circRNAs were calculated by TPM (transcripts per kilobase of exon model per million mapped reads). Clean data per sample exceeds 10 GB. We find 235 significantly up-regulated circRNAs and 271 significantly down-regulated circRNAs in T-BEAS-2B cells relative to C-BEAS-2B cells. Our work provides clues and evidence for exploring the mechanism of circRNAs in cadmium carcinogenesis.

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Integrative transcriptomic of human bronchial BEAS-2B exposed to seasonal urban PM

Project description:The aim of the present study was to characterize a common and unique signalling pathways associated to two seasonal particulate matter (PM) (winter PM2.5 and summer PM10) in human bronchial BEAS-2B cells by mean of a global transcriptomic profiling (including genes and miRNA) and the investigation of selected pathways at protein level.

2016-05-26 | E-MTAB-3630 | biostudies-arrayexpress

Integrative transcriptomic profiling of human bronchial BEAS-2B exposed to seasonal urban PM

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Project description:Proteome analysis of Beas-2B cells with S. pneumoniae D39 after 9 h and 16 h of infection

2023-07-04 | PXD039059 | Pride

Silica-exposed BEAS-2B epithelial cells

Project description:We used mass spectrometry to profile changes in metabolites, proteins, and phosphorylation in silica-exposed BEAS-2B epithelial cells.

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HmiOA4.1 microarrays-BEAS-2B cells treatment with NiCl2

Project description:To investigate the effects of nickel on miRNAs expression in BEAS-2B cells.

2018-04-17 | GSE67997 | GEO

BEAS-2B cells: Control versus chemical exposure

Project description:Transcriptonal profiling of BEAS-2B cells: Control versus skin sensitizers; Control versus respiratory sensitizers; Control versus non-sensitizing irritants

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Next Generation Sequencing Analysis of miRNA profiling in Epithelial Type and Mesenchymal Type Beas-2b Cells

Project description:We reported the application of next generation sequencing technology for high-throughput profiling of miRNA expression in bronchile epithelial cell line Beas-2b with epithelial or mesenchymal mophology. By comparation the expression aboundence of known miRNAs between epithelial type and mesenchymal type Beas-2b cells, we found both upregulated and downregulated miRNAs in bronchial epithelial cells during EMT. This study provides a basic condition for further investigation of the roles of the regulated miRNAs during EMT in bronchial epithelial cells.

2018-12-26 | GSE117522 | GEO

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