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Expression comparison between C. elegans drh-3(ne4253) and wild-type

ABSTRACT: Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA systems. We show that in the germ-line, one system is dependent on worm-specific AGOs, including WAGO-1, which localizes to germ-line nuage structures called P-granules. WAGO-1 silences certain genes, transposons, pseudogenes and cryptic loci. Finally, we demonstrate that components of the nonsense-mediated decay pathway function in at least one WAGO-mediated surveillance pathway. These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest novel regulatory functions for small RNAs.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE18230 | GEO | 2009/10/01

SECONDARY ACCESSION(S): PRJNA123501

REPOSITORIES: GEO

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High throughput sequencing of mutants in the WAGO pathway

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2009-10-01 | E-GEOD-18215 | biostudies-arrayexpress

High throughput sequencing of mutants in the WAGO pathway

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A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing

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Maternal piRNAs are essential for germline development following de-novo establishment of endo-siRNAs in Caenorhabditis elegans

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