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The impact of long-term physical inactivity on adipose tissue immunometabolism

ABSTRACT: RNAsequencing from 20 young healthy male (20-45 years) abdominal subcutaneous adipose tissue before and after 60 days' head-down bed rest.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182303 | GEO | 2022/01/01

REPOSITORIES: GEO

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Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men

Project description:Rationale: Physical inactivity is a risk factor for insulin resistance. We examined the effect of nine days of bed rest on basal and insulin stimulated expression of genes potentially involved in insulin action by applying hypothesis-generating microarray in parallel with candidate gene real-time PCR approaches in 20 healthy, young men. Furthermore, we investigated whether bed rest affected DNA methylation in the promoter region of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene. Subjects were re-examined after four weeks of retraining. Findings: Bed rest induced insulin resistance and altered the expression of more than 4,500 genes. These changes were only partly normalized after four weeks of retraining. Pathway analyses revealed significant down-regulation of 34 pathways, predominantly those of genes associated with mitochondrial function including PPARGC1A. Despite induction of insulin resistance, bed rest resulted in a paradoxically increased response to acute insulin in the general expression of genes, particularly those involved in inflammation and endoplasmatic reticulum (ER) stress. Furthermore, bed rest changed gene expressions of several insulin resistance and diabetes candidate genes. We also observed a trend toward increased PPARGC1A DNA methylation after bed rest. Conclusions: Impaired expression of PPARGC1A and other genes involved in mitochondrial function as well as a paradoxically increased response to insulin of genes involved in inflammation and ER stress may contribute to the development of insulin resistance induced by bed rest. Lack of complete normalization of changes after four weeks of exercise retraining underscores the importance of maintaining a minimum of daily physical activity.

2010-09-21 | GSE24215 | GEO

Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men

2010-09-21 | E-GEOD-24215 | biostudies-arrayexpress

Influence of nine days of physical inactivity on the expression of miRNAs and their predicted target genes in human skeletal muscle

Project description:miRNA profiles were investigated in healthy young males before and after bed rest and subsequently after retraining.

2024-01-22 | GSE86037 | GEO

The Impact of Long-term Physical Inactivity on Adipose Tissue Immunometabolism.

Project description:ContextAdipose tissue and physical inactivity both influence metabolic health and systemic inflammation, but how adipose tissue responds to chronic physical inactivity is unknown.ObjectiveThis work aimed to characterize the impact of chronic physical inactivity on adipose tissue in healthy, young males.MethodsWe collected subcutaneous adipose tissue from 20 healthy, young men before and after 60 days of complete bed rest with energy intake reduced to maintain energy balance and fat mass. We used RNA sequencing, flow cytometry, ex vivo tissue culture, and targeted protein analyses to examine adipose tissue phenotype.ResultsOur results indicate that the adipose tissue transcriptome, stromal cellular compartment, and insulin signaling protein abundance are largely unaffected by bed rest when fat mass is kept stable. However, there was an increase in the circulating concentration of several adipokines, including plasma leptin, which was associated with inactivity-induced increases in plasma insulin and absent from adipose tissue cultured ex vivo under standardized culture conditions.ConclusionPhysical inactivity-induced disturbances to adipokine concentrations such as leptin, without changes to fat mass, could have profound metabolic implications outside a clinical facility when energy intake is not tightly controlled.

| S-EPMC8684473 | biostudies-literature

Extensive and dynamic changes in the human blood transcriptome and its circadian organisation when lying in bed, head down, for 60 days

Project description:We investigated the human 24-h time series blood transcriptome in 20 individuals during a 90-day constant bed rest protocol.

2024-03-09 | GSE253864 | GEO

Effects of a nutritional supplement in older individuals subject to ten days of complete bed rest.

Project description:Older individuals subjected to complete bed rest for 10 days were randomized to a complete nutritional supplement with or without hydroxymethyl butyrate (HMB). Muscle biopsies from the vastus lateralis were obtained immediately prior and at the conclusion of the bed rest. RNA-sequencing was performed to determine the gene expression changes associated with bed rest. Marked decrease in the expression of genes associated with mitochondrial energy metabolism including fatty acid oxidation, TCA cycle and the electron transport chain was observed with bed rest. These effects were partly mitigated in the subjects provided the nutritional supplement with HMB.

2019-11-01 | GSE130722 | GEO

The impact of long-term physical inactivity on adipose tissue immunometabolism

Project description:The impact of long-term physical inactivity on adipose tissue immunometabolism

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Gene expression in skeletal muscle in older individuals subject to ten days of complete bed rest.

Project description:Older individuals were subjected to complete bed rest for 10 days. Muscle biopsies from the vastus lateralis were obtained immediately prior and at the conclusion of the bed rest. RNA-sequencing was performed to determine the gene expression changes associated with bed rest. Marked decrease in the expression of genes associated with mitochondrial energy metabolism including fatty acid oxidation, TCA cycle and the electron transport chain was observed. Upstream regulator analysis revealed that many of these genes are downstream targets of the transcription factors PPAR, ERR and PGC-1alpha.

2019-09-24 | GSE126865 | GEO

Adipose tissue adaptations to an 8-week weight loss intervention in younger and older obese men,

Project description:Abdominal subcutaneous adipose tissue transcriptomes were analyzed between 11 young and 8 elderly obese men during a lifestyle intervention. Lifestyle intervention: Individuals underwent 8-weeks of calorie-restriction of 20% below their daily energy requirement aerobic combined to two sessions of resistance exercise per weeks.

2021-08-23 | GSE159809 | GEO

Human (21d bed rest) Muscle LC-MSMS

Project description:Muscle atrophy is one of the main deleterious consequences of physical inactivity. Bed rest represents a unique model of physical inactivity and results in main changes in muscle metabolic function. However, our knowledge on the detrimental effects of bed rest still needs clarification. The aim of this study was to decipher the mechanisms involved in disuse muscle atrophy and the effects of different countermeasures in healthy men. A cross-over trial was designed, with each trial consisting in a 7-day ambulatory control period for baseline data collection, followed by 21 days head-down tilt bed rest with or without resistive vibration exercise only or combined with nutritional (protein) supplement as countermeasures. The changes in the Vastus lateralis muscle proteome collected in the eight volunteers were characterized using mass spectrometry-based label-free quantitative proteomics.

2020-07-07 | PXD006882 | Pride

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