Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Binding and Transcriptional profile of Halobacterium salinarum histone

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Halobacterium salinarum

PROVIDER: GSE182514 | GEO | 2021/08/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Binding profile of Halobacterium salinarum histone [ChIP-Seq]

Project description:Unlike the characterised histone proteins of a number of methanogenic and themophilic archaea, previous research indicated that HpyA, the sole histone encoded in the model halophile Halobacterium salinarum, is not involved in DNA packaging. Instead, it was found to have widespread but subtle effects on gene expression, and affect cell morphology. Here we report that HpyA is important for growth in reduced salinity and regulates gene expression of ion uptake and nucleotide metabolism pathways in a salt-dependent manner. Analysis of the genome-wide binding pattern of HpyA reveals that its binding is dependent on external salt concentration as well as growth phase. Combining the transcriptomic and ChIP-Seq data suggests a role for HpyA as a regulator of the expression of ion uptake genes as well as a co-regulator of other genes of diverse functions in a salt-dependent manner. In this GEO series , we use ChIP-Seq to study the genome-wide binding of HpyA-HA in optimal (4.2M NaCl) and reduced (3.4M) in exponential and stationary phase, using HA-tag-allone strains as a control

2021-08-25 | GSE182492 | GEO

Transcriptional profile of Halobacterium salinarum histone [RNA-seq]

Project description:Unlike the characterised histone proteins of a number of methanogenic and themophilic archaea, previous research indicated that HpyA, the sole histone encoded in the model halophile Halobacterium salinarum, is not involved in DNA packaging. Instead, it was found to have widespread but subtle effects on gene expression, and affect cell morphology. Here we report that HpyA is important for growth in reduced salinity and regulates gene expression of ion uptake and nucleotide metabolism pathways in a salt-dependent manner. Analysis of the genome-wide binding pattern of HpyA reveals that its binding is dependent on external salt concentration as well as growth phase. Combining the transcriptomic and ChIP-Seq data suggests a role for HpyA as a regulator of the expression of ion uptake genes as well as a co-regulator of other genes of diverse functions in a salt-dependent manner. In this GEO series , we use RNA-Seq to study the transcriptonal effect of HpyA in optimal (4.2M NaCl) and reduced (3.4M) during exponential growth phase.

2021-08-25 | GSE182493 | GEO

Binding and Transcriptional profile of Halobacterium salinarum histone

Project description:Binding and Transcriptional profile of Halobacterium salinarum histone

| PRJNA756548 | ENA

Elucidating microRNA regulatory networks using transcriptional, post-transcriptional and histone modification measurements

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-12-15 | E-GEOD-61035 | biostudies-arrayexpress

TALE transcription factors binding and transcriptional effect

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-31 | E-GEOD-39609 | biostudies-arrayexpress

Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1.

Project description:BackgroundArchaea combine bacterial-as well as eukaryotic-like features to regulate cellular processes. Halobacterium salinarum R1 encodes eight leucine-responsive regulatory protein (Lrp)-homologues. The function of two of them, Irp (OE3923F) and lrpA1 (OE2621R), were analyzed by gene deletion and overexpression, including genome scale impacts using microarrays.ResultsIt was shown that Lrp affects the transcription of multiple target genes, including those encoding enzymes involved in amino acid synthesis, central metabolism, transport processes and other regulators of transcription. In contrast, LrpA1 regulates transcription in a more specific manner. The aspB3 gene, coding for an aspartate transaminase, was repressed by LrpA1 in the presence of L-aspartate. Analytical DNA-affinity chromatography was adapted to high salt, and demonstrated binding of LrpA1 to its own promoter, as well as L-aspartate dependent binding to the aspB3 promoter.ConclusionThe gene expression profiles of two archaeal Lrp-homologues report in detail their role in H. salinarum R1. LrpA1 and Lrp show similar functions to those already described in bacteria, but in addition they play a key role in regulatory networks, such as controlling the transcription of other regulators. In a more detailed analysis ligand dependent binding of LrpA1 was demonstrated to its target gene aspB3.

| S-EPMC2894021 | biostudies-literature

Foxd3 binding at enhancers controls developmental timing of gene expression by regulation of histone acetylation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-12-08 | E-GEOD-58408 | biostudies-arrayexpress

Transcriptional profile of C. elegans following RNAi of elt-2

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-03-22 | E-GEOD-69264 | biostudies-arrayexpress

Transcriptional profile of deiodinase knockdown in zebrafish head, abdomen and tail

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-04-10 | E-GEOD-61625 | biostudies-arrayexpress

Methylation QTLs are associated with coordinated changes in transcription factor binding, histone modifications, and gene expression levels

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series