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Mitochondrial variant enrichment from high-throughput single-cell RNA-seq resolves clonal populations

ABSTRACT: Reconstructing lineage relationships in complex tissues can reveal mechanisms underlying development and disease. Recent methods combine single-cell transcriptomics with mitochondrial DNA variant detection to establish lineage relationships in primary human cells, but are not scalable to interrogate complex tissues. To overcome this limitation, here we develop a technology for high-confidence detection of mitochondrial mutations from high-throughput single-cell RNA-sequencing. We use the new method to identify skewed immune cell expansions in primary human clonal hematopoiesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182685 | GEO | 2021/12/08

REPOSITORIES: GEO

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