Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ICLIP-Seq analyses of NSP12-bound RNAs

ABSTRACT: We known that NSP12 as an important RNA binding protein, but the potential substrate RNA of host cells remain elusive.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182750 | GEO | 2024/08/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

iCLIP-Seq analyses of YTHDF3-bound RNAs

Project description:We found that YTHDF3 work as m1A reader,but the potential substrate RNA remain elusive.

2020-03-24 | GSE135642 | GEO

iCLIP-Seq analyses of DDX46-bound RNAs

Project description:DDX46 is identified to be required at the early step of pre-spliceosome assembly,but the potential roles of DDX46 in RNA editing and whether DDX46 could regulate antiviral innate immunity by editing antiviral transcripts in the nucleus remain elusive. iCLIP-Seq analyses of DDX46-bound RNAs from uninfected and VSV-infected RAW264.7 cells

2016-05-13 | E-GEOD-81386 | biostudies-arrayexpress

iCLIP-Seq analyses of DDX46-bound RNAs

2016-05-13 | GSE81386 | GEO

iCLIP-Seq analyses of NSP12-bound RNAs

Project description:iCLIP-Seq analyses of NSP12-bound RNAs

| PRJNA757705 | ENA

Transcriptome analyses of HEK293T and VERO upon overexpression

Project description:NSP12 as the key RNA transcription polymerase of SARS-CoV-2, we intend to know whether NSP12 regulate the mRNA transcription of host cell upon viral infection

2024-08-22 | GSE182742 | GEO

Translatome analyses of HEK293T upon NSP12 overexpression

Project description:NSP12 of SARS-CoV-2 can recruit the elongation factor eEF1A, we intend to know whether NSP12 regulate the mRNA translation level of host cell upon viral infection

2024-08-22 | GSE182745 | GEO

iCLIP-Seq analyses of YTHDF3-bound RNAs

Project description:iCLIP-Seq analyses of YTHDF3-bound RNAs

| PRJNA559536 | ENA

Identification of Meiotic-P26-bound RNAs in cutlured Drosophila SL2 cells by iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation)

Project description:The TRIM-NHL protein Meiotic P26 (Mei-P26) governs gene expression and controls stem cell fate in Drosophila. Mei-P26 represses the translation of the key regulator protein Nanos and induces miRNA-dependent silencing to regulate stem cell renewal, differentiation and proliferation. The structural and mechanistic details of mRNA target recognition and RNA binding by its C-terminal NHL domain remain elusive. To identify its cellular target RNAs, we performed iCLIP2 experiments in cultured Drosophila SL2 cells that were transfected with Flag-tagged Mei-P26 full-length, or its NHL domain.

2021-06-01 | GSE152013 | GEO

Discovery and functional interrogation of the virus and host RNA interactome of SARS-CoV-2 proteins [RNA-Seq]

Project description:The coronavirus disease 2019 (COVID-19) pandemic was caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Understanding the molecular functions of SARS-CoV-2 proteins is thus imperative to developing effective antiviral treatments. Here, we use enhanced crosslinking and immunoprecipitation to investigate SARS-CoV-2 protein interactions with viral and host RNAs. SARS-CoV-2 proteins, NSP8 and NSP12, are found to specifically bind to untranslated regions of the RNA viral genome, with NSP12 additionally binding to all transcription regulatory sequences. This provides evidence for their central roles in replication and transcription. Moreover, we discovered a potential site of NSP12 mediated genome recombination, which could explain the genetic diversity found in coronaviruses. SARS-CoV-2 proteins exogenously expressed in human lung epithelial cells bind to 4,281 unique host RNAs. Nine SARS-CoV-2 proteins upregulate target gene expression, including NSP12 which upregulates mitochondrial electron transport and N-linked glycosylation proteins. Furthermore, siRNA knockdown of NSP12-targeted proteins in human lung organoid cells demonstrates substantial antiviral effects. Conversely, NSP9 inhibits host gene expression via blocking mRNA export and dampens antiviral inflammation response such as interleukin 1α (IL1α) production. Our extensive viral protein-RNA interactome provides a catalog of potential therapeutic targets and offers insight into the etiology of COVID-19 as a safeguard against future pandemics.

2022-03-09 | GSE173507 | GEO

Discovery and functional interrogation of the virus and host RNA interactome of SARS-CoV-2 proteins [Ribo-Seq]

2022-03-31 | GSE198899 | GEO