Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNA-seq of lung-resident memory B cells and comparison to splenic memory and naive B cells

ABSTRACT: The aim was to utilise bulk RNAseq to compare the transcriptional profiles of memory B cells (Bmem) from the lung tissue to circulatory Bmem derived from spleen, in the context of X31 influenza-induced immune memory. In this study we utilise AID-Cre Rosa-Stop-YFP reporter mice to track previously activated B cell populations, and combine this with an X31-influenza-specific HA probe to isolate flu-specific Bmem. We find that lung Bmem express a number of tissue retention-associated genes (Cd44, Cd69) and downregulate circulatory genes (Sell, S1pr1), in comparison to splenic Bmem. We also identify a number of chemotactic factors (Cxcr3, Ccr1, Ccr5) that are expressed at higher levels in lung Bmem than splenic Bmem.

ORGANISM(S): Mus musculus

PROVIDER: GSE183135 | GEO | 2022/03/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Longitudinal transcriptional profiling of CD8 cells in response to infection and vaccination across tissues

Project description:To determine whether persistent antigen in AdNP immunized mice has any potential cell-intrinsic effects on the genetic program of lung TRM that result in their enhanced longevity, we compared the transcriptional profiles of influenza NP-specific lung TRM (CD8+ i.v. antibody- NP+ CD69+ CD103+), lung TRM that were CD69 single positive (CD8+ i.v. antibody- NP+ CD69+ CD103-) and splenic TEM (CD8+ CD62L- NP+) from mice either infected with x31 influenza or immunized with AdNP at 1-month (35 days post-infection (d.p.i.), x31 and AdNP) and 1-year (365 d.p.i., AdNP only) time points.

2022-08-17 | GSE198980 | GEO

Heterotypic Influenza Infections Mitigate Susceptibility to Secondary Bacterial Infection

Project description:Influenza associated bacterial super-infections have devastating impacts on the lung and can result in increased risk of mortality. New strains of influenza circulate throughout the population yearly promoting the establishment of immune memory. Nearly all individuals have some degree of influenza memory prior to adulthood. Due to this we sought to understand the role of immune memory during bacterial super-infections. An influenza heterotypic immunity model was established using influenza A/PR/8/34 and A/X31. We report here that influenza experienced mice are more resistant to secondary bacterial infection with methicillin-resistant Staphylococcus aureus as determined by wasting, bacterial burden, pulmonary inflammation, and lung leak, despite significant ongoing lung remodeling. Multidimensional flow cytometry and lung transcriptomics revealed significant alterations in the lung environment in influenza-experienced mice compared with naïve animals. These include changes in the lung monocyte and T cell compartments, characterized by increased expansion of influenza tetramer specific CD8+ T cells. The protection that was seen in memory experienced mouse model is associated with the reduction in inflammatory mechanisms making the lung less susceptible to damage and subsequent bacterial colonization. These findings provide insight into how influenza heterotypic immunity re-shapes the lung environment and the immune response to a re-challenge event, which is highly relevant to the context of human infection.

2022-06-27 | GSE206095 | GEO

Transcriptional responses of antigen-specific CD8 T cells after secondary influenza virus challenge

Project description:To evaluate the transcriptional responses of effector and memory virus-specific CD8 T cell subsets after primary and secondary influenza infection, we sorted subsets of FluNP 366-374/Db+ CD8+ T cells from the BAL, lung, and spleen at the peak of primary x31 influenza infection (day 10), resting memory (day 30), or 3 days after secondary PR8 influenza challenge (Day 30+3). Cells from the circulation were excluded from BAL and lung based on intravital labeling with anti-CD45. Memory cells from the lung were further separated in TRM and TEM subsets based on expression of CD69 at the day 30 and day 30+3 timepoints. Memory cells from the BAL were separated into long-term airway resident versus recently-recruited cells based on CD11a expression at the day 30 and days 30+3 timepoints. The results show that virus-specific BAL and lung TRM cells, but not lung TEM cells or spleen TEM cells, rapidly alter their transcriptional program and increase expression of effector molecules within 3 days of a secondary influenza challenge.

2024-02-15 | GSE247464 | GEO

Host response in the lung to influenza infection with PR8, VN or X31 strains at 12, 16 and 24 hours

Project description:Array analysis of total lung RNA obtained from mice 12,16,24 h post infection with influenza. Strains used were Mock, PR8, X31, VN62 (1x10^5pfu) Time Point: 12, 16, 24 h post infection (Mock, PR8, X31, VN62); Mock group has three biological replicates, virally infected groups have nine replicates.

2013-03-31 | E-GEOD-42285 | biostudies-arrayexpress

SBRI_AA Host response in the lung to influenza infection with PR8, VN or X31 strain at 12 hours.

Project description:Array analysis of total lung RNA obtained from mice 12 h post infection with influenza. Strains used were Mock, PR8, X31, VN6+2 (1x10^5pfu) Time Point: 12 h post infection (Mock, PR8, X31, VN); Mock group has four biological replicates, virally infected groups have five replicates. Total samples: 19.

2012-09-26 | E-GEOD-37245 | biostudies-arrayexpress

Host response in the lung to influenza infection with PR8, VN or X31 strains at 12, 16 and 24 hours

Project description:Array analysis of total lung RNA obtained from mice 12,16,24 h post infection with influenza. Strains used were Mock, PR8, X31, VN62 (1x10^5pfu)

2013-03-31 | GSE42285 | GEO

SBRI_AA Host response in the lung to influenza infection with PR8, VN or X31 strain at 12 hours.

Project description:Array analysis of total lung RNA obtained from mice 12 h post infection with influenza. Strains used were Mock, PR8, X31, VN6+2 (1x10^5pfu)

2012-09-26 | GSE37245 | GEO

The PD-1 pathway regulates development and function of memory CD8+ T cells following respiratory viral infection

Project description:The PD-1:PD-L co-inhibitory pathway regulates dysfunctional T cells in chronic viral infection and cancer, but the role of this pathway in effector and memory responses following acute infection or vaccination remains less clear. Here we demonstrated that in the absence of signals from the PD-1 pathway, cell intrinsic alterations during initial CD8+ T cell priming resulted in excessive early CD8+ T cell expansion, but increased CD8+ T cell contraction and aberrant effector to memory CD8+ T cell transition. Overall, our studies revealed a critical and previously unappreciated role for PD-1 as an integrator of early CD8+ T cell activation signals that promoted optimal CD8+ T cell memory formation and durability. This novel PD-1 function has therapeutic implications for the generation of T cell memory during PD-1 cancer immunotherapy and modulation of the PD-1 pathway to enhance immune memory following acute infection or prophylactic vaccination. Microarrays were performed on influenza-specific CD8+ T cells isolated from the lungs of wildtype and PD-L1/L2 double knockout mice that were infected intranasally with X31-GP33. Influenza-specific CD8+ T cells were harvested at day 8 in order to determine the global program of gene expression at during the effector phase of the response to influenza to begin to dissect the role of PD-1 signaling early during effector and memory differentiation.

2020-06-30 | GSE149425 | GEO

Transcriptional response in influenza-infected lungs from C57BL/6 wt, 129S7 wt and IFNAR-/- (129) mice

Project description:We used microarrays to analyse the global program of gene expression in response to Influenza A (X31) infection in lungs from C57BL/6 wt, 129S7 wt and IFNAR-/- (129) mice. Mock- or 5 day influenza A (X31)-infected total lungs from mice with the indicated genotypes were collected and processed for expression profiling.

2014-04-30 | E-GEOD-55403 | biostudies-arrayexpress

Transcriptional control of influenza-specific CD4+ tissue-resident memory T cell generation

Project description:Tissue-resident memory T cells (TRM) are a non-circulating subset of memory that are maintained at sites of pathogen entry and mediate optimal protection against reinfection. Lung TRM can be generated in response to respiratory infection or vaccination, however, the molecular pathways involved in CD4+TRM establishment have not been defined. Here, we performed transcriptional profiling of influenza-specific lung CD4+TRM following influenza infection to identify pathways implicated in CD4+TRM generation and homeostasis. Lung CD4+TRM displayed a unique transcriptional profile distinct from spleen memory, including up-regulation of a gene network induced by the transcription factor IRF4, a known regulator of effector T cell differentiation.

2020-09-01 | GSE137386 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data