Transcriptomics

Dataset Information

0

Rad21 is the core subunit of cohesin complex in directing genome organization


ABSTRACT: In eukaryotes, the nuclear chromatin in the interphase is a hierarchical organization. Previous studies have suggested that cohesin-DNA interactions play important architectural functions for chromatin structure in the interphase. In this study, we applied super-resolution imaging, single-molecule imaging to measure the distribution of cohesin subunits. Hi-C and RNA-seq were used to reveal the 3D genome structures alterations and the relationship with breast cancer upon Rad21 up-regulation. Hi-C experiment showed that the contact frequency of short distance was increased while that of long distance was decreased in the absence of Rad21 up-regulation. Chromatin interactions between A and B compartments increased and compartmentalization strength was reduced significantly upon Rad21 up-regulation. Correspondingly, accumulated contacts were shown at TAD corners and inter-TAD interactions increased. These observations support cohesin loop extrusion model and lead us to propose the unique role of Rad21-loader interaction in cohesin loading and further cohesin extrusion. Moreover, we combined transcriptome changes and chromatin structure alterations upon Rad21 up-regulation and revealed the correlation between Rad21 and breast cancer by affecting chromatin architecture.

ORGANISM(S): Homo sapiens

PROVIDER: GSE183186 | GEO | 2022/05/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-02-24 | E-GEOD-63515 | biostudies-arrayexpress
2024-07-28 | GSE273257 | GEO
2023-07-28 | GSE196727 | GEO
2023-07-28 | GSE196449 | GEO
2023-07-28 | GSE196034 | GEO
2024-08-04 | GSE248280 | GEO
2024-08-04 | GSE248281 | GEO
2015-02-24 | E-GEOD-62904 | biostudies-arrayexpress
2019-10-03 | PXD014896 | Pride
2013-12-16 | E-GEOD-44267 | biostudies-arrayexpress