Transcriptomics

Dataset Information

0

Combinatorial transcription factor profiles predict mature and functional human islet α and β cells


ABSTRACT: Islet-enriched transcription factors (TFs) exert broad control over cellular processes in pancreatic α and β cells and changes in their expression are associated with developmental state and diabetes. However, the implications of heterogeneity in TF expression across islet cell populations are not well understood. To define this TF heterogeneity and its consequences for cellular function, we profiled >40,000 cells from normal human islets by scRNA-seq and stratified α and β cells based on combinatorial TF expression. Subpopulations of islet cells co-expressing ARX/MAFB (α cells) and MAFA/MAFB (β cells) exhibited greater expression of key genes related to glucose sensing and hormone secretion relative to subpopulations expressing only one or neither TF. Moreover, all subpopulations were identified in native pancreatic tissue from multiple donors. By Patch-seq, MAFA/MAFB co-expressing β cells showed enhanced electrophysiological activity. Thus, these results indicate combinatorial TF expression in islet α and β cells predicts highly functional, mature subpopulations.

ORGANISM(S): Homo sapiens

PROVIDER: GSE183568 | GEO | 2021/09/09

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-11-04 | E-MTAB-3070 | biostudies-arrayexpress
2023-05-01 | GSE230728 | GEO
2013-11-20 | E-GEOD-52522 | biostudies-arrayexpress
2013-11-20 | GSE52522 | GEO
2020-03-23 | GSE145347 | GEO
2021-09-10 | GSE183561 | GEO
2023-08-16 | GSE232750 | GEO
| PRJNA824305 | ENA
2022-06-24 | GSE200355 | GEO
| PRJNA761336 | ENA