Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential gene expression of Wnt agonist (BIO and lithium chloride) treated human dental pulp stem cells

ABSTRACT: Small molecules, BIO and Lithium chloride are widely used to activate Wnt signaling. It has been shown that these molecules induced beta-catenin accumulation and translocation, leading to the activation of Wnt signaling. These molecules also control various cell responses. Here, the differential gene expression of human dental pulp stem cells treated with BIO and Lithium chloride was examined using a high throughput RNA sequencing technique. Results demonstrated that BIO and Lithium chloride regulated the mRNA expression of various genes in human dental pulp stem cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE183633 | GEO | 2022/08/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Differential gene expression of Wnt agonist (BIO and lithium chloride) treated human dental pulp stem cells

Project description:Differential gene expression of Wnt agonist (BIO and lithium chloride) treated human dental pulp stem cells

| PRJNA761431 | ENA

The effects of antimigratory drugs lithium chloride and 6-bromoindirubin-3'-oxime (BIO) in glioblastoma cells

Project description:The purpose of the experiment was to better understand the effects of antimigratory drugs lithium chloride and 6-bromoindirubin-3'-oxime (BIO) in glioblastoma cells. U251 cells were grown to 60-70% confluency in DMEM containing 10% FBS in 6 well plates. Drugs were added (LiCl 20 mM, BIO 5 micromolar, with carrier controls; either NaCl or DMSO) and incubated for 24 hours. Cells were then harvested and RNA purified using TRIZOL.

2023-03-20 | E-MTAB-12775 | biostudies-arrayexpress

Differential gene expression of Wnt ligands/Wnt agonist treated human dental pulp stem cells

Project description:Wnt regulates various cell responses. In dental pulp cells, Wnt signaling control cell proliferation, apoptosis, migration and differentiation. Here, the differential gene expression of human dental pulp stem cells treated with Wnt ligands or Wnt agonist was examined using a high throughput RNA sequencing technique. Results demonstrated that Wnt ligands or Wnt agonist altered numerous gene expression in human dental pulp stem cells.

2022-12-09 | GSE183444 | GEO

Differential gene expression in Lithium chloride (LiCl) treated Scleroderma (SSc) skin fibroblasts

Project description:Differential gene expression in Lithium chloride (LiCl) treated Scleroderma (SSc) skin fibroblasts

| PRJNA1071564 | ENA

Bio-ball vs mesoball

Project description:MIXL1-GFP reporter lines (HESC3MIXL1-GFP/w and MEL1MIXL1-GFP/w, referred to as HES3 and MEL1 in this submission) were differentiated as spin embryonic bodies (Ebs) supplemented with mesodermal inducing growth factors (BMP4,SCF, VEGF based). Methylcellulose hematopoietic colony forming assays were performed by culturing dissociated day 4 EBs in the presence of hematopoietic growth factors (VEGF, SCF, IL3, IL6, TPO, EPO and FLT3L) for 7-9 days. Addition of WNT3a to the methylcellulose lead to the formation of compact, mesodermal colonies, which we term 'mesosphere' (Meso-balls). We demonstrated that the inclusion of WNT3a in the methylcellulose could be replaced with BIO, a GSK3 inhibitor, acting as a canonical WNT signaling agonist. Mesospheres formed in the culture supplemented with BIO molecules were termed 'BIO-balls'. Expression profiling between 'meso-balls' and 'BIO-balls' were compared and analyzed for markers assisiting in defining their phenotypes.

2012-12-01 | E-MEXP-3738 | biostudies-arrayexpress

Adipose-derived stromal/stem cells for bio-root regeneration

Project description:To explore molecular mechanisms of different seed cells in bio-root regeneration, RNA sequencing (RNA-seq) was performed on adipose-derived stromal/stem cells (ASCs) and two dental derived stem cells

2022-05-15 | GSE203020 | GEO

Gene expression profiling of lithium stimulated human mesenchymal stem cells

Project description:Wnt signaling in essential for osteoblast differentiation and is activated by lithium via inhibition of glycogen synthase kinase 3beta. Although lithium has been shown to enhance osteoblast differentiation in mice models and accelerate bone repair, its effect in humans is not clear. Therefore, we performed gene expression profiling of human MSCs from 3 donors treated with lithium (5mM) for 7 days. Microarray profiling of lithium-stimulated hMSC revealed decreased expression of adipogenic genes (CEBPA, CMKLR1, HSD11B1) and genes involved in lipid biosynthesis. Interestingly, osteoclastogenic factors and immune responsive genes (IL7, IL8, CXCL1, CXCL12, CCL20) were also downregulated. Negative transcriptional regulators of the osteogenic program (TWIST1 and PBX1) were suppressed while genes involved in mineralization like CLEC3B and ATF4 were induced. These results suggest suppression of adipogenic program and induction of osteogenic genes.

2013-02-02 | GSE34747 | GEO

Time course single-cell multiome sequencing data from mESCs treated with Activin A/Lithium Chloride

Project description:Time course single-cell multiome sequencing data from mESCs treated with Activin A/Lithium Chloride

| PRJNA924750 | ENA

Gene expression profiling of lithium stimulated human mesenchymal stem cells

2013-02-02 | E-GEOD-34747 | biostudies-arrayexpress

Transcription profiling of galactose-grown S. cerevisiae cells exposed to lithium chloride

Project description:Wild type strain CEN.PK113-7D was grown in an aerobic batch cultivation with a start concentration of 20 g/L galactose. During exponential growth at a biomass concentration of 3 g dry weight/L LiCl was added to a concentration of 10 mM. Just before addition of LiCl (time 0) and 20, 40, 60 and 140 minutes after addition of LiCl samples were taken for transcription analysis. Lithium inhibits phosphoglucomutase whereby both galactose uptake and growth is strongly affected.

2007-07-07 | E-GEOD-461 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data